Fig 1: Immunofluorescence staining of ACTB, ACTG, PFN1, MYLK, MYL12B, FGF1, IBSP, SPP1, COL11A2, and COL9A2 in human degenerated IVD tissue. Scale bar = 20 µm. DAPI, 4,6-diamino-2-phenyl indole (DAPI) field; Field 1, red fluorescent field stained with different antibodies; Merge1, Field1 merged with DAPI field; Merge2, bright field merged with Merge1. Representative images from N = 7 patients
Fig 2: Expression pattern of ACTG1, TNC and ACTB in control and VWM cortices. (A–F) Immunohistochemistry against ACTG1, TNC and ACTB in control cortex (upper panel) and VWM cortex (bottom panel). Insets in (E,F) are higher magnification images, showing cellular immunoreactivity of ACTB in VWM cortex, but not in control cortex. Blue stain indicates nuclei. Scale bars: 20 µm. Depicted images are stains of cortical tissue of control 2 and VWM 1.
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