Anti-gamma Actin antibody [2A3] ab123034 from Abcam

Product Specs
ItemAnti-gamma Actin antibody [2A3]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab123034
Antibody TypePrimary
Conjugate/TagUnconjugated
IsotypeIgG2b
Quantity100 UL
ApplicationsImmunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Immunohistochemistry paraffin embedded sections
ClonalityMonoclonal
Clone2A3
HostMouse, Nude mouse
NCBI Gene AliasesACT, ACTG, DFNA20, DFNA26, HEL-176
Concentration1 mg/ml
Research AreaNeuroscience
Gene NameACTG1
ReactivityHuman, Pig
Targetgamma Actin

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Polo-like kinase-1 mediates hepatitis C virus-induced cell migration, a drug target for liver cancerLife Science AllianceNovember 1, 2023Korri E El-Khobar, Enoch Tay, Eve Diefenbach, Brian S Gloss, Jacob George, Mark W Douglaswas purchased from Thermo Fisher Scientific (#37-7100); γ-actin antibody was purchased from Abcam (#ab123034). HCV NS5A sheep polyclonal antibody and NS5A mouse antibody were gifts from Professor Mark Harris (University

(2) Significance of platelet adhesion-related genes in colon cancer based on non-negative matrix factorization-based clustering algorithmDIGITAL HEALTHSeptember 26, 2023Xiao-jv Chi, Yi-bei Song, Deng-he Liu, Li-qiang Wei, Xin An, Zi-zhen Feng, Xiao-hua Lan, Dong Lan, Chao Huangwith the appropriate primary antibody (POU6F1, PA5-61403, Invitrogen; MATN3, ab238893, Abcam; ACTG1, ab123034, Abcam; ALOX12, ab211506, Abcam) diluted in PBS. After overnight incubation at 4°C, the slides were

(3) Cortical Pathology in Vanishing White MatterCellsNovember 12, 2022Man JHK, van Gelder CAGH, Breur M, Okkes D, Molenaar D, van der Sluis S, Abbink T, Altelaar M, van der Knaap MS, Bugiani M.*,- GFAPδ,1:500,†,- Nestin,1:200,Millipore,MAB5623 GJA1,1:100,Sigma-Aldrich,C6219 GLUL,1:5000,ThermoFisher,PA5-81505 HRSP12,1:100,ThermoFisher,PA5-54622 ACTG1,1:100,Abcam,ab123034 TNC,1:200,Abcam,ab108930 ACTB,1:1500,Abcam,ab8226 S100β,1:100,DAKO,Z311 List of antibodies used

(4) Cervical Cancer Cells-Derived Extracellular Vesicles Containing microRNA-146a-5p Affect Actin Dynamics to Promote Cervical Cancer Metastasis by Activating the Hippo-YAP Signaling Pathway via WWC2Journal of oncologyJanuary 1, 2022Wang W, Wu L, Tian J, Yan W, Qi C, Liu W, Xuan S, Shang A.Abcam), p-cofilin (1 : 1000, ab12866, Abcam), F-actin (1 : 1000, ab205, Abcam), G-actin (1 : 1000, ab123034, Abcam), and GAPDH (1 : 5000, ab8245, Abcam) as well as horseradish peroxidase labeled goat anti-rabbit

(5) Actin gamma 1 is a critical regulator of pancreatic ductal adenocarcinomaSaudi Journal of GastroenterologyJanuary 1, 2022Yichen Tang, Yichen Tang, Xiaobing Huang, Xiaobing Huangbuffer (pH 6.0) for antigen retrieval. The sections were then incubated with ACTG1 antibody (1:300, #ab123034, Abcam) overnight at 4°C, followed by sequential incubation with secondary antibodies and diaminobenzidine.

(6) Transcriptional profiling of intervertebral disc in a post-traumatic early degeneration organ culture modelJOR spineSeptember 1, 2021Cui S, Zhou Z, Chen X, Wei F, Richards RG, Alini M, Grad S, Li Z.MA5‐27738, Thermofisher), MYLK (1:100, PA5‐79716, Thermofisher), OPN (1:1000, ab8448, Abcam), ACTG (1:500, ab123034, Abcam), PFN1 (1:100, ab124904, Abcam), MYL12B (1:250, ab137063, Abcam), ACTB (1:100, P60709, Cell Signaling),

(7) Nujiangexanthone A Inhibits Hepatocellular Carcinoma Metastasis via Down Regulation of Cofilin 1Frontiers in Cell and Developmental BiologyMarch 12, 2021Li Zhang et al.incubated in different primary antibodies: CFL1 (CST #5175), F–actin (Abcam#ab130935), G–actin (Abcam#ab123034), GAPDH (Abcam#ab128915). All antibodies were used at a 1:1,000 dilution, except for GAPDH, which was

(8) Reciprocal regulation of Aurora kinase A and ATIP3 in the control of metaphase spindle lengthCellular and molecular life sciences : CMLSFebruary 1, 2021Nehlig A, Seiler C, Steblyanko Y, Dingli F, Arras G, Loew D, Welburn J, Prigent C, Barisic M, Nahmias C.(T9026; Abcam, 1/5000e), antiphospho-histone H3 (06-570; Millipore, 1/1000e), mouse anti-gamma-actin (ab123034; Abcam, 1/5000e), mouse antivinculin (V9264; Sigma, 1/5000e). NC O 137 Proteomics and mass spectrometry MCF-7 cells

(9) Cardiac pericytes function as key vasoactive cells to regulate homeostasis and diseaseFEBS open bioJanuary 1, 2021Lee LL, Khakoo AY, Chintalgattu V.,1 : 1000,WB caldesmon,Abcam,ab32330,1 : 1000,WB vimentin,Abcam,ab92547,1 : 1000,WB calponin,Abcam,ab46794,1 : 1000,WB y‐SMA,Abcam,ab123034,1 : 1000,WB SMMHC11,Abcam,ab53219,1 : 1000,WB MLC2,Cell Signaling Technology,3672S,1 : 1000,WB Vinculin,Invitrogen,700062,1 : 1000

(10) Heat exposure impairs porcine oocyte quality with suppressed actin expression in cumulus cells and disrupted F-actin formation in transzonal projectionsJournal of Animal Science and BiotechnologyDecember 1, 2020Chao Yin, Jie Liu, Zhanglin Chang, Bin He, Yang Yang, Ruqian Zhao-polyacrylamide gel electrophoresis (PAGE). Mouse anti-β-actin antibody (AP0060, Bioworld, 1:200) and mouse anti-γ-actin antibody (ab123034, Abcam, 1:200) were used as primary antibodies. Western blot analyses were carried out according to

(11) Mechanism of actin N-terminal acetylationScience advancesApril 1, 2020Rebowski G, Boczkowska M, Drazic A, Ree R, Goris M, Arnesen T, Dominguez R.anti–Ac-β-actin (monoclonal mouse, 1:5000; Abcam, ab6276), anti–Ac-γ-actin (monoclonal mouse, 1:5000; Abcam, ab123034), anti–pan-actin (polyclonal rabbit, 1:2000; Cell Signaling Technology, 4968), anti-V5 (monoclonal mouse,

(12) NR4A1 promotes TNF‑α‑induced chondrocyte death and migration injury via activating the�AMPK/Drp1/mitochondrial fission pathwayInternational Journal of Molecular MedicineNovember 8, 2019Zhibo Zheng, Shuai Xiang, Yingjie Wang, Yulei Dong, Zeng Li, Yongbo Xiang, Yanyan Bian, Bin Feng, Bo Yang, Xisheng Weng1:1,000), complex I subunit NDUFB8 (CI-20; Abcam; cat. no. ab110242; 1:1,000), G-actin (Abcam; cat. no. ab123034; 1:1,000), F-actin (Abcam; cat. no. ab205; 1:1,000), AMPK (Abcam; cat. no. ab131512; 1:1,000) and phoshpo-AMPK

(13) Ubiquitin-proteasomal regulation of chromatin remodeler INO80 in the nucleus accumbens mediates persistent cocaine cravingScience advancesOctober 1, 2019Werner CT, Mitra S, Martin JA, Stewart AF, Lepack AE, Ramakrishnan A, Gobira PH, Wang ZJ, Neve RL, Gancarz AM, et al.Santa Cruz Biotechnology, sc-136363), anti-EGR1 (1:100; Santa Cruz Biotechnology, sc-515830), anti–γ-actin (1:1000; Abcam, ab123034), anti–pan-SHANK [1:500; University California (UC) Davis/National Institutes of Health (NIH) NeuroMab

(14) RRAD suppresses the Warburg effect by downregulating ACTG1 in hepatocellular carcinomaOncoTargets and therapyJanuary 1, 2019Yan Y, Xu H, Zhang L, Zhou X, Qian X, Zhou J, Huang Y, Ge W, Wang W.rabbit polyclonal antibody (ab75100; Abcam, Cambridge, UK) and anti-ACTG1 mouse monoclonal antibody (ab123034; Abcam) were used as the primary antibodies for immunohistochemical staining; the staining was performed

(15) Mannan rich fraction from yeast modulates inflammatory responses in intestinal cells (HT-29) exposed toEscherichia coliJournal of Applied Animal NutritionJanuary 1, 2019Niall Browne, Aimee Traynor, Karina A. HorganTICAM-1 (Santa Cruz, SC514384) and, for housekeeping, protein actin using Mouse Anti-human Actin (Abcam, AB123034). Each primary antibody was individually incubated for 1 h at RT. Secondary Horseradish Peroxidase (HRP)

(16) miR-125a induces apoptosis, metabolism disorder and migration impairment in pancreatic cancer cells by targeting Mfn2-related mitochondrial fissionInternational Journal of OncologyApril 26, 2018Lichao Pan, Lin Zhou, Weijia Yin, Jia Bai, Rong Liuab56788; 1:1,000), mitochondrial fission 1 protein (Fis1; cat. no. ab71498; 1:1,000), G-actin (cat. no. ab123034; 1:1,000) and F-actin (cat. no. ab205; 1:1,000) from Abcam (Cambridge, MA, USA); and complex III subunit

(17) Zearalenone regulates endometrial stromal cell apoptosis and migration via the promotion of mitochondrial fission by activation of the JNK/Drp1 pathwayMolecular Medicine ReportsMarch 29, 2018Huixiang Wang, Xiaoli Zhao, Chengxiang Ni, Yuyang Dai, Yan GuoCST Biological Reagents Co., Ltd.) associated X protein (Bax; 1:2,000; ab32503), γ‑actin (1:1,000; ab123034) and F‑actin (1:1,000; ab205; all Abcam) overnight at 4˚C (30). The membranes were washed in Tris‑buffered

(18) Capillary pericytes mediate coronary no-reflow after myocardial ischaemiaeLifeNovember 9, 2017Fergus M O'Farrell, Svetlana Mastitskaya, Matthew Hammond-Haley, Felipe Freitas, Wen Rui Wah, David Attwellactin (mouse monoclonal) Abbiotec 251815 (1:100) antibody anti-gamma actin (mouse monoclonal) AbCam ab123034 (1:100) antibody anti-glycophorin A (mouse monoclonal) AbCam ab9520 (1:2000) antibody anti-neutrophil

(19) Chimeric antibody targeting SRPK-1 in the treatment of non-small cell lung cancer by inhibiting growth, migration and invasionMolecular Medicine ReportsFebruary 1, 2017Fan Wu, Jie Li, Xin Du, Weisan Zhang, Ping Lei, Qiang Zhangmouse anti‑human primary antibody: SRPK‑1 (ab50927; 1:500), Cytochalasin‑D (ab143484; 1:500), G‑actin (ab123034; 1:500) and β‑actin (ab5694; 1:500; all from Abcam, China) for 12 h at 4˚C. All samples were washed

(20) Characteristic expression and significance of CCL19 in different tissue types in chronic rhinosinusitisExperimental and therapeutic medicineJanuary 1, 2016Zou Y, Wang Y, Wang SB, Kong YG, Xu YU, Tao ZZ, Chen SM.of 1:500 (0.2 µg/ml) for 2 h at room temperature. Mouse anti-β-actin monoclonal antibody (1:1,000; ab123034) was used as a loading control. A horseradish peroxide (HRP)-conjugated secondary antibody (1:1,000;

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Anti-gamma Actin antibody [2A3] from Abcam

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Anti-gamma Actin antibody [2A3] from Abcam

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