Fig 1: miR-185-5p represses laryngeal cancer cell proliferation and motion by CST1. (a) Number of colonies formed by TU686 and TU177 cells after transfections of NC mimics, miR-185-5p mimics, or cotransfection with miR-185-5p mimics+pcDNA-CST1. (b, c) Migrated and invaded TU686 and TU177 cells after transfections were photographed and counted. ***P < 0.001.
Fig 2: Representative immunofluorescence of CILP (red), CST1 (red), DPP4 (red), F2RL2 (green), PITX2 (green), RIOX (red), ZFHX4 (green) in esophageal squamous cell carcinoma (ESCC) samples and normal tissues of 112 ESCC patients from Zhongshan Hospital, Fudan University.
Fig 3: CST1 knockdown reduced laryngeal cancer cell proliferation and motion. (a) RT-qPCR analysis of CST1 expression in TU686 and TU177 cells after transfection with sh-CST1#1/2. (b) Number of colonies formed by sh-CST1#1/2-transfetced TU686 and TU177 cells. (c, d) Migrated and invaded TU686 and TU177 cells after transfection with sh-CST1#1/2 were photographed and counted. ***P < 0.001.
Fig 4: LINC01278 inhibits TU686 and TU177 cell proliferation and motion by the miR-185-5p/CST1 axis. (a) Number of colonies formed by TU686 and TU177 cells after transfections of sh-NC, sh-LINC01278, or cotransfections with sh-LINC01278+miR-185-5p inhibitor, sh-LINC01278+pcDNA-CST1. (b, c) Number of migrated and invaded TU686 and TU177 cells after transfections. ***P < 0.001.
Fig 5: CST1 shows upregulation in laryngeal cancer cells. (a) RT-qPCR analysis of CST1 expression in TU686, M4E, TU177, TU138, and control HuLa-PC cells. (b) Western blotting of CST1 protein in TU686, M4E, TU177, TU138, and control HuLa-PC cells. (c) Immunofluorescence staining of CST1 in TU177, TU138, and control HuLa-PC cells. *P < 0.05, **P < 0.01, and ***P < 0.001.
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