Fig 1: In vivo detection of two MSP limb bud subpopulations based on MSC-am localization. (A) Transversal section of an E10.5 mouse embryo to show the histological organization at this stage; the immunodetections of CD29, SCA-1, CD44, CD105, CD90, and CD73 markers in E10.5 limb buds are shown. The limb bud for each MSC-am marker corresponds to the region inside the square in panel (A). (B) Dot plots of the multiparametric flow cytometry analysis showing the hierarchy to identify sSca+ and sSca– subpopulations in E10.5, E11.5, and E12.5 mouse limbs. The number of positive cells for each subpopulation is expressed in percentage; ±mean standard deviation. (C) Comparison of the expression percentage of the sSca+ subpopulation between E10.5, E11.5, and E12.5 limbs. Statistical significance was set as follows: E10.5 vs. E11.5 p > 0.0061; 10.5 vs. 12.5 p > 0.0023; E11.5 vs. E12.5 p > 0.4079, **p < 0.005. NT, neural tube; AGM, aorta-gonad-mesonephros; Do, dorsal; V, ventral; Pr, proximal; D, distal. In (A) AER is marked with an asterisk. The scale bar is set at 200 µm and is representative for all images.