Fig 2: Invasive breast carcinomas (IBCs) are characterized by deregulation of all proteins PIWIL1-2-3-4. At magnification ×200.

Fig 3: YTHDC2 interacts with several other proteins involved in RNA regulation.(A, B) Immunoprecipitation with α-YTHDC2 from P12 and P18 wild-type mouse testis extracts containing either an RNase Inhibitor (-RNase) or RNase A (+RNase). Western blots probed with α-YTHDC2, α-MEIOC, α-PABPC1, α-FMR1, α-CAPRIN1, α-PTBP1, α-EWS, α-MOV10, α-YBX1, α-G3BP2 and α-UPF1. (C-H) Immunofluorescence images of testis cross-sections from P18 (C-E) control (+/+; Ythdc2flox/∆) and (F-H) Ythdc2 cKO (UBC-CreERT2; Ythdc2flox/∆) mice stained for MEIOC (green) and SYCP3 (red). Scale bars: 50 µm. (D, E) High-magnification images of (D) pachytene spermatocytes in boxed region 1 in panel Cʹ and (E) leptotene spermatocytes in boxed region 2 in panel Cʹ. (G, H) High-magnification image of (G) pachytene spermatocytes in boxed region 1 in panel Fʹ and (H) leptotene spermatocytes in boxed region 2 in panel Fʹ. Arrows: MEIOC protein in perinuclear puncta in pachytene spermatocytes. (I-Iʹʹʹ) High-magnification immunofluorescence images of spermatocytes from a P18 Ythdc2 cKO mouse testis cross-section stained for MEIOC (green), MIWI (blue) and DCP1A (red). Arrows: puncta in the cytoplasm of pachytene spermatocytes. See also Figure 4 – source data 1 and Figure 4 – source data 2.