Fig 2: TNX protein level in tumoral versus normal tissues.Cancers of high incidence/mortality were studied (upper part), as well as cancers for which TNX status was previously studied (lower part) thanks to TNX immunolabelling of tissue sections on Tissue MicroArray slides (#MC6163 and #T392a). A: Representative cores of normal (left) and tumoral (right) tissues. Scale bar = 100 µm. B: Quantification of stromal TNX staining intensity. A segmentation between stromal and epithelial areas was performed, followed by measurement of reciprocal mean grey value (staining intensity) in the stromal area. Segmentation training was newly perfomed for each spot. Mann-Whitney test was performed between normal and tumoral conditions (****p < 0.0001; ***p < 0.001; **p < 0.01 and *p < 0.05). C: Clinical sample scoring. Staining was blindly scored according to 4 categories: negative, low, intermediate and high. Results are presented as percentage of total cores for each cancer type.

Fig 3: Variation of TNXB mRNA expression in tumors versus normal or adjacent tissues.Cancers of high incidence/mortality were studied (A and C), as well as cancers for which TNX status had been previously studied (B and D). A and B: Expression values of TNXB mRNA obtained from the selected datasets extracted from the GEO database. Non-parametric paired (breast, stomach, liver, and kidney cancers) and unpaired (lung, prostate, colon, glioma, ovary, melanoma and leiomyoma cancers) tests were performed comparing tumoral to normal tissues. For datasets comparing TNXB mRNA expression in tumoral sample versus adjacent normal tissue, a bar chart is presented just below the first graphical representation. Each bar corresponds to a patient and is either blue, when TNXB mRNA expression is downregulated in tumoral sample or red, when TNXB expression is upregulated. C and D: Expression data and associated p-values extracted from the UALCAN web-portal. Numbers of tumoral and control samples for each dataset are indicated. ****p < 0.0001; ***p < 0.001; **p < 0.01 and *p < 0.05.