Fig 1: Absence of CD138-positive (CD138+ve) plasma cells in Nfkb2-/- and RelB-/- intestinal lamina propria. Small intestine from C57BL/6 and Nfkb2-/- mice (males and females) and RelB-/- females (along with their wild-type and heterozygous littermates) were fixed and 4 µm sections were used for staining for CD138 (×40 magnification). A: representative images of stained villi of the four genotypes are presented. Tissues were counterstained with hematoxylin. B: comparison of CD138+ve plasma cells numbers between C57BL/6 (black diamonds) and the respective wild-type littermate controls [Nfkb2+/+ (black circles) or RelB+/+ (black squares)]. C: effect of Nfkb2 deficiency on lamina propria CD138+ve plasma cell numbers in Nfkb2+/+ (black circles), Nfkb2+/- (black and white circles), and Nfkb2-/- (open circles) samples. D: effect of RelB deficiency on lamina propria CD138+ve plasma cell numbers in RelB+/+ (black squares), RelB+/- (black and white squares), and RelB-/- (open squares) samples. Ten villi per mouse were counted (n = 3–7 mice/genotype). Kruskal–Wallis multiple-comparison test was applied. *P < 0.05. Scale bars represent 100 µm.