Fig 1: Nedd4-2 was not required for mTORC2 regulation on the plasma membrane trafficking of PepT2 and transport activity of PepT2 in BMEC. (A) Representative western blots are shown for PepT2 in the membrane of scramble, Rictor or Rictor + Nedd4-2 silenced BMEC. Upper panel: representative blot; bottom panel: quantitative representation. (B) The uptake of ß-Ala-Lys-AMCA transfected with scramble, Rictor or Rictor + Nedd4-2 siRNA. Values are the means ± SD (n = 3). Means without a common letter differ (P < 0.05). Nedd4-2 = neural precursor cell expressed developmentally downregulated 4 isoform 2; mTORC2 = mammalian target of the rapamycin complex 2; PepT2 = peptide transporter 2; BMEC = bovine mammary epithelial cells; Rictor = rapamycin-insensitive companion of mTOR; ß-Ala-Lys-AMCA = ß-Ala-Lys-N-7-amino-4-methylcoumarin-3-acetic acid.
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