Goat anti-Rabbit IgG (H+L), DyLight 633 35562 from Thermo Fisher Scientific

Product Specs
ItemGoat anti-Rabbit IgG (H+L), DyLight 633
CompanyThermo Fisher Scientific
Price Pricing Info Supplier Page View Company Product Page
Catalog Number35562
Quantity1 mL
ApplicationsFlow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
FormatPBS with 1% BSA and 0.02% sodium azide; pH 7.4
Conjugate/TagDyLight™ 633
ClonalityPolyclonal
ReactivityRabbit (Rb)
Concentration1 mg/mL
Storage Temperature4° C
ImmunogenPurified Rabbit IgG, whole molecule
Storage4° C
PurityAntigen affinity chromatography
IsotypeIgG
Brand/TrademarkABR (now Thermo Scientific Pierce Antibodies)
Antibody TypeSecondary
HostGoat
TargetRabbit IgG (H+L)

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Thermo Fisher Scientific

81 Wyman Street

Waltham, MA 02451

United States

Phone: 800-678-5599
Fax: 800-331-2286

Company Profile

Website: www.thermofisher.com

(1) Breast Cancer Stem Cell–Derived Tumors Escape from γδ T-cell Immunosurveillance In Vivo by Modulating γδ T-cell LigandsCancer Immunology ResearchJune 2, 2023Katrin Raute, Juliane Strietz, Maria Alejandra Parigiani, Geoffroy Andrieux, Oliver S. Thomas, Klaus M. Kistner, Marina Zintchenko, Peter Aichele, Maike Hofmann, Houjiang Zhou, et al.Southern Biotech. Anti-γδTCR-PE (clone SA6.E9), anti-γδTCR-FITC (clone SA6.E9), anti-rabbit-DyLight633 (polyclonal, 35562), anti–PD-1-PE-Cy7 (clone J105), streptavidin-eFluor450 (48-4317-82), and streptavidin-PE-Cy7 (SA1012)

(2) The Lipid Raft-Associated Protein Stomatin Is Required for Accumulation of Dectin-1 in the Phagosomal Membrane and for Full Activity of Macrophages against Aspergillus fumigatusmSphereFebruary 21, 2023Marie Goldmann, Franziska Schmidt, Zoltán Cseresnyés, Thomas Orasch, Susanne Jahreis, Susann Hartung, Marc Thilo Figge, Marie von Lilienfeld-Toal, Thorsten Heinekamp, Axel A. BrakhagevATPase) or 1:100 (dectin-1). The incubation with secondary antibodies, goat anti-rabbit IgG DyLight 633 (35562; Thermo Fisher Scientific), goat anti-rabbit IgG Alexa Fluor 532 (A11009; Life Technologies), and donkey

(3) PAI-1 is a potential transcriptional silencer that supports bladder cancer cell activityScientific ReportsDecember 1, 2022Hideki Furuya et al.Cells were washed with PBS and subsequently treated with anti-rabbit DyLight 633 antibody (Catalog #: 35562; Thermo Fisher Scientific, Waltham, MA, 1:280 dilution) at room temperature for 1 h in the dark. Chamber

(4) The steroid hormone estriol (E3) regulates epigenetic programming of fetal mouse brain and reproductive tractBMC BiologyDecember 1, 2022Yuping Zhou et al.without the ERα insert was used as a control (Invitrogen). Flag-ERβ were obtained from Addgene (plasmid #35562). (4 × 107) Ishikawa cells and (4 × 106) SH-SY5Y cells were treated with transfection mix [Lipofectamine

(5) Conservation and divergence of myelin proteome and oligodendrocyte transcriptome profiles between humans and miceeLifeMay 11, 2022Gargareta VI, Reuschenbach J, Siems SB, Sun T, Piepkorn L, Mangana C, Späte E, Goebbels S, Huitinga I, Möbius W, et al.donkey anti-mouse Alexa 555 (Invitrogen #A31570, 1:1000) and goat anti-rabbit DyLight 633 (Invitrogen #35562, 1:500). The labeled sections were imaged using the confocal microscope LSM880 (Zeiss, Oberkochen, Germany).

(6) White matter integrity in mice requires continuous myelin synthesis at the inner tongueNature communicationsMarch 4, 2022Meschkat M, Steyer AM, Weil MT, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Phan NTN, Ruhwedel T, Sadowski B, et al.donkey-α-Mouse Alexa 555, Invitrogen Cat# A31570, 1:1000; goat-α-rabbit Dylight 633, Invitrogen Cat# 35562, 1:500. Immunohistochemistry For Caspr1, Nav 1.6, and MBP labeling, mice were anesthetized with Avertin77

(7) Isolation and immunofluorescence staining of Aspergillus fumigatus conidia-containing phagolysosomesSTAR ProtocolsMarch 19, 2021Marie Goldmann, Franziska Schmidt, Irene Kyrmizi, Georgios Chamilos, Axel A. Brakhage#ab73404) was used as primary antibody in a 1:500 dilution. Goat-anti-rabbit DyLight633 (Invitrogen, #35562) was used as secondary antibody in a 1:200 dilution. Arrows indicate isolated phagolysosomes. ∗ indicates

(8) Cell Adhesion Molecules Involved in Neurodevelopmental Pathways Implicated in 3p-Deletion Syndrome and Autism Spectrum DisorderFrontiers in Cellular NeuroscienceJanuary 13, 2021Josan Gandawijaya, Rosemary A. Bamford, J. Peter H. Burbach, Asami Oguro-Andopatient with intellectual disability. Am. J. Med. Genet. Part A 158A, 2587–2590. doi: 10.1002/ajmg.a.35562 Rogers, D. C., Fisher, E. M. C., Brown, S. D. M., Peters, J., Hunter, A. J., and Martin, J. E. (1997).

(9) EpCAM-Mediated Cellular Plasticity Promotes Radiation Resistance and Metastasis in Breast CancerFrontiers in Cell and Developmental BiologyJanuary 6, 2021Arijit Mal, Amirali B. Bukhari, Ram K. Singh, Aastha Kapoor, Amlan Barai, Ishan Deshpande, Tabassum Wadasadawala, Pritha Ray, Shamik Sen, Abhijit Decoverslips is incubated with a secondary antibody, which is tagged with anti-rabbit Dylight 633 (Invitrogen 35562) (dilution 1:100) for 1 h at room temperature. The primary antibody used is γH2AX (Cell Signaling Technology,

(10) Microglia facilitate repair of demyelinated lesions via post-squalene sterol synthesisNature NeuroscienceJanuary 1, 2021Stefan A. Berghoff et al.anti-goat Jackson Immuno Research #705-165-147, dilution 1:500; Dylight633 donkey anti-rabbit Invitrogen #A-35562, dilution 1:1000). For chromogenic stainings, blocking of endogenous peroxidase activity with 3% hydrogen

(11) Light organ photosensitivity in deep-sea shrimp may suggest a novel role in counterilluminationScientific ReportsDecember 1, 2020Heather D. Bracken-Grissom, Danielle M. DeLeo, Megan L. Porter, Tom Iwanicki, Jamie Sickles, Tamara M. Frankidentified from RNAseq. The secondary used was goat anti-rabbit DyLight633 (Thermofisher, Catalog number: 35562). A control was run in parallel lacking primary antibodies to address possible non-specific binding

(12) Flotillin-Dependent Membrane Microdomains Are Required for Functional Phagolysosomes against Fungal InfectionsCell reportsAugust 18, 2020Schmidt F, Thywißen A, Goldmann M, Cunha C, Cseresnyés Z, Schmidt H, Rafiq M, Galiani S, Gräler MH, Chamilos G, et al.647,Cell Signaling Technology,4414,1:500 Goat anti-rabbit IgG DyLight 633,Thermo Fisher Scientific,35562,1:200 to 1:500 Goat anti-rabbit Abberior Star Red,Abberior GmbH,STRED,1:100 Goat anti-mouse Alexa

(13) The prevalence and prognostic significance of estrogen receptor beta expression in non-small cell lung cancerTranslational lung cancer researchJune 1, 2020Enwere EK, Dean ML, Li H, D'Silva A, Bebb DG.were transiently transfected with either 0.3, 0.4 or 0.5 µg/mL pcDNA-FLAG-ERβ plasmid (catalog number 35562, Addgene, Cambridge, MA, USA), or an empty vector expressing green fluorescent protein (pcDNA-GFP, kindly

(14) The meiotic TERB1-TERB2-MAJIN complex tethers telomeres to the nuclear envelopeNature communicationsFebruary 4, 2019Wang Y, Chen Y, Chen J, Wang L, Nie L, Long J, Chang H, Wu J, Huang C, Lei M.(Thermo, 35502, 35553, 1:500), DyLight 550 (Thermo, 84540, 84541, 1:500), DyLight 633 (thermos, 35512, 35562, 1:500). Quantitative real-time PCR Total RNA was purified from seminiferous tubules using Trizol reagent

(15) Biomimetic artificial organelles with in vitro and in vivo activity triggered by reduction in microenvironmentNature CommunicationsDecember 1, 2018T. Einfalt, D. Witzigmann, C. Edlinger, S. Sieber, R. Goers, A. Najer, M. Spulber, O. Onaca-Fischer, J. Huwyler, C. G. Palivanfollowed by staining with the secondary goat anti-rabbit polyclonal Dylight633-labelled antibody (1:1000; #35562, Thermo Fisher Scientific). Cell nuclei were counterstained for 10 min using Hoechst 33342 (0.5 µg ml−1).

(16) The choroid plexus epithelium as a novel player in the stomach-brain axis during Helicobacter infectionBrain, Behavior, and ImmunityMarch 1, 2018N. Gorlé et al.0.1% Tween20) and 1/300 diluted goat anti-rabbit DyLight633 secondary antibody (Thermo Scientific; 35562) diluted in 5% goat serum for 90 min at room temperature. Finally, a Hoechst staining was performed

(17) Treatment with the herbal formula Songyou Yin inhibits epithelial-mesenchymal transition in hepatocellular carcinoma through downregulation of TGF-β1 expression and inhibition of the SMAD2/3 signaling pathwayOncology LettersApril 1, 2017Susu Zheng, Qingan Jia, Hujia Shen, Xin Xu, Jiajia Ling, Chuyu Jing, Boheng Zhangincubation with a fluorescein isothiocyanate-labeled goat anti-rabbit immunoglobulin G antibody (cat. no. 35562; dilution, 1:200; Thermo Fisher Scientific, Inc.) at room temperature for 30 min. The fixed cells were

(18) Analysis of Phagosomal Antigen Degradation by Flow OrganellocytometryBio-protocolNovember 20, 2016Hoffmann E, Pauwels AM, Alloatti A, Kotsias F, Amigorena S.Goat anti-rabbit IgG (H+L) antibody, DyLight 633 conjugate (Thermo Fisher Scientific, Invitrogen™, catalog number: 35562) Goat anti-rabbit IgG (H+L) antibody, Alexa Fluor 568 conjugate (Thermo Fisher Scientific, Invitrogen™,

(19) Combined 2-deoxy glucose and metformin improves therapeutic efficacy of sodium-iodide symporter-mediated targeted radioiodine therapy in breast cancer cellsBreast cancer (Dove Medical Press)January 1, 2015Chatterjee S, Thaker N, De A.Abcam (Cambridge, UK). H2A.X monoclonal antibody (MA515130) and anti-mouse (35512) and anti-rabbit Dylite633 (35562) antibody were obtained from Thermo Fisher Scientific. Enliten ATP assay kit (FF2000) was from Promega Breast Cancer

(20) The anti-migratory effects of FKBPL and its peptide derivative, AD-01: regulation of CD44 and the cytoskeletal pathwayPloS oneJanuary 1, 2013Yakkundi A, McCallum L, O'Kane A, Dyer H, Worthington J, McKeen HD, McClements L, Elliott C, McCarthy HO, Hirst DG, et al.migration, intercellular communication, and vascular tube formation by thromboxane A (2). J Biol Chem 274: 35562–35570. 20. McCallum L, Price S, Planque N, Perbal B, Pierce A, et al. (2006) A novel mechanism for BCR-ABL

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Goat anti-Rabbit IgG (H+L), DyLight 633 from Thermo Fisher Scientific

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Goat anti-Rabbit IgG (H+L), DyLight 633 from Thermo Fisher Scientific

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