Fig 1: CPSN substrate supported the differentiation of primary BADSCs towards cardiomyocytes. (a) Immunostaining images showing the specific markers CD90 and UCP1 of BADSCs. (b) Flow cytometry analysis showing the surface markers CD29 and CD44 of the isolated BADSCs. (c) Representative images showing the tri-lineage differentiation capacity of the primary BADSCs. (d–f) Representative immunofluorescent images showing the expression of MEF2C and cTnT, and the relative fluorescence intensity was quantified accordingly. (g–i) RT-PCR analysis showing the expression level of cardiomyogenesis-related genes VE-cadherin, T-box transcription factor 5 (Tbx5), and NK2 homeobox 5 (Nkx2.5). (j–l) Representative pseudo-color images showing Fluo-4 fluorescence intensity in BADSCs cultured on CPSN and PSN and in rCMs, and the fluorescence intensity was quantified accordingly. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001 according to one-way ANOVA followed by LSD post-hoc test (e-i, k, and l). All data were shown as mean ± SD. Values of independent experiments were: n = 3 (e, f and l), 5 (g–i). Twenty-five individual cells were counted (k).