Fig 1: Inactivation of AMPK/FOXO1 signaling reversed the impacts of LKB1 overexpression on inflammation and GnRH expression in HG- and HF-induced GT1-7 cells. Detection of LKB1 (A) mRNA and (B) protein expression using RT-qPCR and western blot analysis. (C) RT-qPCR was performed to examine GnRH expression. (D) Measurement of ERß, CD36 and GPR54 expression using western blot analysis. **P<0.01 and ***P<0.001. AMPK, AMP-activated protein kinase; FOXO1, forkhead box protein O1; LKB1, live kinase B1; GnRH, gonadotropin-releasing hormone; HF, high fat; HG, high glucose; RT-qPCR, reverse transcription-quantitative qPCR; ERß, estrogen receptor-ß; CD36, cluster of differentiation 36; GPR54, G-protein-coupled receptor; Oe, overexpression; NC, negative control.
Fig 2: Graph to show mRNA expression of KISS1 and KISS1R as determined by qRT-PCR. Data are expressed as mean±s.e.m. arbitrary units calculated following normalisation of copy number using normalisation factor obtained from the three housekeeping genes YWHAZ, HPRT and SDHA.
Fig 3: LKB1 overexpression inhibits GnRH and PP-related protein expression in HG- and HF-induced GT1-7 cells. (A) RT-qPCR was performed to examine GnRH expression. (B-C) Measurement of ERß, CD36 and GPR54 expression using western blot analysis. **P<0.01 and ***P<0.001. LKB1, live kinase B1; GnRH, gonadotropin-releasing hormone; PP, precocious puberty; HF, high fat; HG, high glucose; RT-qPCR, reverse transcription-quantitative qPCR; ERß, estrogen receptor-ß; CD36, cluster of differentiation 36; GPR54, G-protein-coupled receptor; Oe, overexpression; NC, negative control.
Fig 4: (a) Photomicrographs to show placental expression of KISS1 and KISS1R in early pregnancy, normal term pregnancy and PE. Staining was predominantly localised to syncytiotrophoblast cells (indicated with open headed arrow) with a small amount of staining present in villous cytotrophoblast cells (indicated with diamond headed arrow). Positive labelled cells appear brown; magnification ×200. (b) Graph of immunohistochemical labelling for KISS1 and KISS1R. Data are expressed as mean±s.e.m. positivity.
Supplier Page from Abcam for Anti-GPR54 antibody