Fig 1: The expression of USP18 protein in EN and LN DLBCL patients. (A) The expression of USP18 protein in EN and LN DLBCL by IHC staining. (B) The H-score of USP18 in tumor tissues of EN and LN DLBCL. (C) The expression of CD83 protein in EN and LN DLBCL by IHC staining. (D) The immunofluorescence double labeled staining of USP18 and CD83 in DLBCL tissues.
Fig 2: The immune gene sets related to USP18 in EN DLBCL. (A) The coexpression heatmap of USP18 with immune gene sets in DLBCL. (B) The linear regression to show the correlation between USP18 and aDCs; (C) The linear regression to show the correlation between USP18 and type I IFN response; (D) The linear regression to show the correlation between USP18 and Tregs; Abbreviations: aDCs, Activated dendritic cells; Tregs, Regulatory T cells.
Fig 3: USP18 silencing suppressed the proliferation and induced apoptosis in human cervical cancer cells. a & b siUSP18–1 or siUSP18–2 significantly suppressed the proliferation of Caski and SiHa cells, n = 3 for each group. * p < 0.05 vs siNC; *** p < 0.001 vs siNC. c The apoptosis of Caski and SiHa cells were upregulated after transfecting with siUSP18–1 or siUSP18–2, n = 3 for each group. *** p < 0.001 vs siNC. d & e Western blot was used to examine the protein contents of Ki-67, Cyclin D1, cleaved PARP, BAX and ß-catenin in Caski and SiHa cells that transfected with siUSP18–1 or siUSP18–2 respectively, n = 3 for each group. *** p < 0.001 vs siNC. The full-length gels are presented in Supplementary Figure 3D-E. f & g Western blot was used to examine the protein contents of USP18, Cleaved caspase-3, AKT and p-AKT in Caski and SiHa cells that transfected with siUSP18–1 or siUSP18–2 respectively, n = 3 for each group. *** p < 0.001 vs siNC. The full-length gels are presented in Supplementary Figure 3F-G
Fig 4: USP18 is upregulated in human cervical cancer tissues. a USP18 is upregulated in primary cervical cancer samples. Data collected from the TCGA database CESC dataset. *** p < 0.001 vs Normal. b The relative mRNA levels of USP18 were much higher in human cervical cancer tissues than that in para-cancer tissues, n = 30 for each group. *** p < 0.001 vs Normal. c USP18 was enriched in the PI3/AKT pathway. d The relative protein levels of USP18 and AKT phosphorylation were upregulated in human cervical cancer tissues compared with that in para-cancer tissues. *** p < 0.001 vs para-carcinoma tissues. n = 4 for each group. The full-length gels are presented in Supplementary Figure 1D. e IHC staining assay indicated the positive correlation between USP18 and AKT phosphorylation in human cervical cancer tissues, n = 30 for each group
Fig 5: Silencing of USP18 inhibited tumorigenicity of human cervical cancer cells in vivo. a & b the tumor volume and weight in nude mice injected with Caski cells transfected with siNC and siUSP18. *** p < 0.001 vs siNC. N = 5 for each group. c TUNEL staining assay was performed to examine the apoptosis of siNC and siUSP18 tumours. *** p < 0.001 vs siNC. Three replications were analysed for each analysis. d Western blot was used to examine the protein levels of USP18, p-AKT, AKT, ß-catenin in siNC and siUSP18 tumours. *** p < 0.001 vs siNC. Three replications were analysed for each analysis. The full-length gels are presented in Supplementary Figure 5D
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