Fig 1: NOV-Primed HSPCs Adopt a Distinct Cell State(A and B) Histogram of fluorescence (A) and median fluorescence intensity (MFI) (B) of DCFDA in 90+RA- cells in STF or NOV+STF; n = 4; mean + SEM; p (STF versus NOV+STF) = 0.062 (t test).(C and D) Flow cytometry histogram (C) and MFI (D) of c-Myc staining in CD34+90+ cells in either STF or NOV+STF. n = 4; mean + SEM; p (STF versus NOV+STF) = 0.02.(E) GSEA of RNA-seq profiles of 90+RA- cells cultured in either STF or NOV+STF, showing reduced transcripts associated with oxidative phosphorylation and MYC and E2F targets (p = 0.00). FDR, false discovery rate; NES, normalized enrichment score.(F) Expression by RNA-seq of indicated genes in 90+RA- cells cultured in either STF or NOV+STF. The fpkm value for STF-treated cells is normalized to 1.0; n = 2.