APC anti-BrdU 339808 from BioLegend

Product Specs
ItemAPC anti-BrdU
CompanyBioLegend
Price Pricing Info Supplier Page View Company Product Page
Catalog Number339808
Conjugate/TagAPC
CloneBu20a
HostMouse
Quantity100 tests
ApplicationsIntracellular Staining for Flow Cytometry (ICFC)
IsotypeIgG1, Kappa
Antibody TypePrimary
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
ClonalityMonoclonal
TargetBrdU

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Diverging regulation of Bach2 protein and RNA expression determine cell fate in early B cell responseCell reportsJuly 5, 2022Hu Q, Xu T, Zhang M, Zhang H, Liu Y, Li HB, Chen C, Zheng J, Zhang Z, Li F, et al.646404, RRID:AB_2563005" anti- IRF4,BioLegend,"Cat# 646414, RRID:AB_2728480" anti- BrdU,BioLegend,"Cat# 339808, RRID:AB_10895898" anti- Bcl6,BD Biosciences,"Cat# 563363, RRID:AB_2738159" anti- Blimp1/Prdm1,BD Biosciences

(2) Anti-PD-1 Checkpoint Therapy Can Promote the Function and Survival of Regulatory T CellsJournal of immunology (Baltimore, Md. : 1950)November 15, 2021Vick SC, Kolupaev OV, Perou CM, Serody JS.#100722), PD1 (RMP1-30 #109103), LAP-TGFβ (TW7-16B4 #141405), CD25 (PC61 #102051), and BrdU (Bu20a #339808) were purchased from BioLegend. Monoclonal antibodies against murine Bcl-2 (3F11 #556537) were purchased

(3) An IRF4–MYC–mTORC1 Integrated Pathway Controls Cell Growth and the Proliferative Capacity of Activated B Cells during B Cell Differentiation In VivoThe Journal of ImmunologyOctober 1, 2021Dillon G. Patterson, Anna K. Kania, Madeline J. Price, James R. Rose, Christopher D. Scharer, Jeremy M. Bossstains were used: B220-PE-Cy7 (Biolegend; 103222), B220-A700 (Biolegend; 103232), BrdU-APC (Biolegend; 339808), c-MYC-PE (Cell Signaling; 14819), c-MYC-Alexa Fluor 647 (Cell Signaling; 13871), CD11b-APC-Cy7 (Biolegend;

(4) GLUT1 Expression in Tumor-Associated Neutrophils Promotes Lung Cancer Growth and Resistance to RadiotherapyCancer researchMay 1, 2021Ancey PB, Contat C, Boivin G, Sabatino S, Pascual J, Zangger N, Perentes JY, Peters S, Abel ED, Kirsch DG, et al.REA798, Miltenyi Biotec, Cat# 130–112-172, RRID:AB_2653444), anti-BrdU-APC (clone Bu20a, BioLegend, 339808, RRID:AB_10895898), anti-PD-L1-BV-785 (clone 10F.9G2, BioLegend, 124331, RRID:AB_2629659). Samples were

(5) MMB-FOXM1-driven premature mitosis is required for CHK1 inhibitor sensitivityCell reportsMarch 2, 2021Branigan TB, Kozono D, Schade AE, Deraska P, Rivas HG, Sambel L, Reavis HD, Shapiro GI, D'Andrea AD, DeCaprio JA.AB_210547 VINCULIN,Sigma-Aldrich,Cat# V9264; RRID: AB_10603627 anti-BrdU-APC conjugate,Biolegend,Cat# 339808; RRID: AB_10895898 anti-mouse IgG - HRP,Bethyl Laboratories,Cat# A90–116P; RRID: AB_67183 anti-rabbit

(6) Replication Stress Induces ATR/CHK1-Dependent Nonrandom Segregation of Damaged ChromosomesMolecular cellMay 21, 2020Xing M, Zhang F, Liao H, Chen S, Che L, Wang X, Bao Z, Ji F, Chen G, Zhang H, et al.

(7) SPHK2-Generated S1P in CD11b⁺ Macrophages Blocks STING to Suppress the Inflammatory Function of Alveolar MacrophagesCell reportsMarch 24, 2020Joshi JC, Joshi B, Rochford I, Rayees S, Akhter MZ, Baweja S, Chava KR, Tauseef M, Abdelkarim H, Natarajan V, et al.E50–2440),BD Bioscience,Cat# 552126; RRID: AB_394341 Anti-Mouse BrdU (Clone BU20-a),BioLegend,Cat# 339808; RRID: AB_10895898 Rabbit Polyclonal anti-TMEM 173/STING,Proteintech,Cat# 19851–1-AP; RRID: AB_10665370 C-terminal

(8) Myeloid differentiation factor 88 signaling in donor T cells accelerates graft-versus-host diseaseHaematologicaJanuary 1, 2020Matsuoka S, Hashimoto D, Kadowaki M, Ohigashi H, Hayase E, Yokoyama E, Hasegawa Y, Tateno T, Chen X, Aoyama K, et al.

(9) The Oncogenic Kaposi's Sarcoma-Associated Herpesvirus Encodes a Mimic of the Tumor-Suppressive miR-15/16 miRNA FamilyCell reportsDecember 3, 2019Morrison K, Manzano M, Chung K, Schipma MJ, Bartom ET, Gottwein E.incubated with 5 μL APC-anti-BrdU diluted in 95 uL PBS containing 0.1% Triton X-100 (Biolegend, Cat No. 339807) for 30 minutes in the dark at room temperature. Cells were then washed once in PBS + 0.5% BSA, and

(10) A distinct transition from cell growth to physiological homeostasis in the tendoneLifeSeptember 19, 2019Mor Grinstein, Heather L Dingwall, Luke D O'Connor, Ken Zou, Terence Dante Capellini, Jenna Lauren GallowayBrdU analysis, cells were stained with anti-BrdU following tendon tissue dissociation (Biolegend, Cat# 339808), and tendons from Scx-Cre;TdTom+; or Scx-Cre;TdTom+;Scx-GFP+ mice were used to analyze TdTom+ or GFP+

(11) Treg Cells Promote the SREBP1-Dependent Metabolic Fitness of Tumor-Promoting Macrophages via Repression of CD8⁺ T Cell-Derived Interferon-γImmunityAugust 20, 2019Liu C, Chikina M, Deshpande R, Menk AV, Wang T, Tabib T, Brunazzi EA, Vignali KM, Sun M, Stolz DB, et al.AB_493314" TNFα,Biolegend,"Clone ID: MP6-XT22, Cat# 506322, RRID: AB_961434" BrdU,Biolegend,"Clone ID: Bu20a, Cat# 339808, RRID: AB_10895898" Arg1,R&D Systems,Cat# IC5868F; RRID: AB_10718118 Foxp3,Thermo Fisher Scientific,"Clone

(12) 53BP1 Supports Immunoglobulin Class Switch Recombination Independently of Its DNA Double-Strand Break End Protection FunctionCell reportsAugust 6, 2019Sundaravinayagam D, Rahjouei A, Andreani M, Tupiņa D, Balasubramanian S, Saha T, Delgado-Benito V, Coralluzzo V, Daumke O, Di Virgilio M.Alexa488,Invitrogen,"Cat# A-11029, RRID: AB_2534088" Mouse anti-BrdU APC antibody (Clone Bu20a),BioLegend,Cat# 339808 RRID:AB_10895898 Mouse anti-HA probe antibody (F-7),Santa Cruz,Cat# sc-7392 RRID:AB_627809 Rabbit

(13) Differentiation of human pluripotent stem cells into neurons or cortical organoids requires transcriptional co-regulation by UTX and 53BP1Nature NeuroscienceMarch 1, 2019Xiaoyang Yang et al.primary antibody for 30 min in FACS buffer and washed in PBS. Antibodies APC anti-BrdU (BioLegend, #339808, 1:500 dilution) and anti-pS10-H3-Alexa488 (Cell Signaling Technology, #3465S, 1:1000 dilution) were

(14) C-Reactive Protein Promotes the Expansion of Myeloid Derived Cells With Suppressor FunctionsFrontiers in immunologyJanuary 1, 2019Jimenez RV, Kuznetsova V, Connelly AN, Hel Z, Szalai AJ.finally BrdU incorporation was probed using of APC conjugated anti-BrdU antibody (clone Bu20a, BioLegend 339808) for 30 min at 4°C (4 μl/tube). Ten minutes prior to cell cytometry total DNA was stained with 1 μg

(15) Intrathymic Notch3 and CXCR4 combinatorial interplay facilitates T-cell leukemia propagationOncogeneDecember 1, 2018Ferrandino F, Bernardini G, Tsaouli G, Grazioli P, Campese AF, Noce C, Ciuffetta A, Vacca A, Besharat ZM, Bellavia D, et al.post-IP-injection, anti-CD4/anti-CD8/anti-Notch3 extracellular staining was followed either by anti-BrdU (BioLegend; 339807) [57] or fixed and permeabilized for intracellular antigen detection of BV510mouse Aati-Ki-67(BD-563462). In silico analysis

(16) Permanent neuroglial remodeling of the retina following infiltration of CSF1R inhibition-resistant peripheral monocytesProceedings of the National Academy of Sciences of the United States of AmericaNovember 27, 2018Paschalis EI, Lei F, Zhou C, Kapoulea V, Dana R, Chodosh J, Vavvas DG, Dohlman CH.PFA, permeabilized with 0.5% Triton-X100 (Sigma-Aldrich), and stained with APC anti-BrdU antibody (no. 339807; Biolegend) for evaluation of BrdU incorporation by flow cytometry. Microglia Depletion. Microglia

(17) A CD103⁺ Conventional Dendritic Cell Surveillance System Prevents Development of Overt Heart Failure during Subclinical Viral MyocarditisImmunityNovember 21, 2017Clemente-Casares X, Hosseinzadeh S, Barbu I, Dick SA, Macklin JA, Wang Y, Momen A, Kantores C, Aronoff L, Farno M, et al.

(18) Targeting Aberrant p70S6K Activation for Estrogen Receptor–Negative Breast Cancer PreventionCancer Prevention ResearchNovember 1, 2017Xiao Wang, Jun Yao, Jinyang Wang, Qingling Zhang, Samuel W. Brady, Banu Arun, Victoria L. Seewaldt, Dihua YuBrij-35/PBS. Stain cells in 100 µl 0.1% Brij-35/PBS with anti-BrdU-APC conjugated antibody (Biolegend #339807, 1:100) for 30 min at room temperature, wash cells with 0.1% Brij-35/PBS, and then stain with propidium

(19) Exogenous remodeling of lung resident macrophages protects against infectious consequences of bone marrow-suppressive chemotherapyProceedings of the National Academy of Sciences of the United States of AmericaOctober 11, 2016Kamei A, Gao G, Neale G, Loh LN, Vogel P, Thomas PG, Tuomanen EI, Murray PJ.

(20) NF-κB-induced microRNA-31 promotes epidermal hyperplasia by repressing protein phosphatase 6 in psoriasisNature communicationsJuly 3, 2015Yan S, Xu Z, Lou F, Zhang L, Ke F, Bai J, Liu Z, Liu J, Wang H, Zhu H, et al.min after cells were washed with PBS. Finally, cells were stained with APC-anti-BrdU Ab (BioLegend, #339808), resuspended in propidium iodide (Life Technologies, #S10274) buffer and analysed with a BD FACSCanto

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APC anti-BrdU from BioLegend

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APC anti-BrdU from BioLegend

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