Fig 1: ADINR RNA Is Required for the Active Chromatin State of the C/EBPa and ADINR Loci(A) ChIP assay of H3K4me3 and H3K27me3 at the C/EBPa and ADINR loci. The qPCR data are presented as the mean ± SD in six independent experiments. *p < 0.05; **p < 0.01. NC, negative control.(B) RNA immunoprecipitation (IP) of endogenous WDR5, PTIP, Menin, and CXXC1 in human adipocytes after 3 days of differentiation. The retrieved RNA was quantified by qRT-PCR (negative controls: U1 and GAPDH RNAs). Approximately 2 µg of cell lysate was loaded as the input control. Error bars represent the mean ± SD in three independent experiments.(C) Immunoblot analysis after RNA pull-down assay of in-vitro-transcribed ADINR RNA and histone2 mRNA.See also Figure S3.