Dystroglycan Antibody AF6868 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemDystroglycan Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberAF6868
ReactivityHuman
TargetDystroglycan
IsotypeIgG
HostSheep
NCBI Gene AliasesDAG, MDDGA9, 156DAG, AGRNR, A3a, MDDGC9, MDDGC7, LGMDR16
ApplicationsWestern Blot, Immunohistochemistry
Quantity100 ug (also 25 ug)
Conjugate/TagUnconjugated
NCBI Full Gene Namedystroglycan 1
ClonalityPolyclonal
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ImmunogenMouse myeloma cell line NS0-derived recombinant human Dystroglycan, Gln28-Val749, Accession # Q14118
ConcentrationLYOPH
PurityAntigen Affinity-purified
Brand/TrademarkR&D Systems, a Bio-Techne Brand
ValidationKnockout Validated
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Unconjugated

Specificity: Detects human Dystroglycan in direct ELISAs and Western blots

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Efficacy of exon-skipping therapy for DMD cardiomyopathy with mutations in actin binding domain 1Molecular Therapy - Nucleic AcidsDecember 12, 2023Naoko Shiba, Xiao Yang, Mitsuto Sato, Shin Kadota, Yota Suzuki, Masahiro Agata, Kohei Nagamine, Masaki Izumi, Yusuke Honda, Tomoya Koganehira, et al.RT. Primary antibodies were rabbit mouse anti-dystrophin (Abcam 15277: 1:3000), sheep anti-DG (R&D Systems AF6868; 1:3,000), rabbit anti-α-actin (HUABIO, Woburn, MA, 0407-3; 1:3,000), mouse anti-desmin [Y66] (Abcam

(2) CRISPR-Cas9 KO Cell Line Generation and Development of a Cell-Based Potency Assay for rAAV-FKRP Gene TherapyCellsOctober 12, 2023Marine Geoffroy, Louna Pili, Valentina Buffa, Maëlle Caroff, Anne Bigot, Evelyne Gicquel, Grégory Rouby, Isabelle Richard, Romain Fragnoud05-593-I, 1:1000 dilution and DSHB, 1:50 dilution), anti-DG core (R&D Systems, Minneapolis, MN, USA, AF6868, 1:1000 dilution), and antibody against β-DG (DSHB, MANDAG2 (7D11), 1:1000 dilution) overnight at 4

(3) Identification of Matriglycan by Dual Exoglycosidase Digestion of α-DystroglycanBIO-PROTOCOLSeptember 20, 2023Ishita Chandel, Kevin P. Campbell(Vector Laboratories, catalog number: AL-1023-10)Core DG antibody (Bio-Techne, R&D systems, catalog number: AF6868)Anti-matriglycan antibody (DSHB, catalog number: IIH6 C4)SOC medium (Invitrogen, One Shot, catalog number:

(4) N-terminal domain on dystroglycan enables LARGE1 to extend matriglycan on α-dystroglycan and prevents muscular dystrophyeLifeFebruary 1, 2023Hidehiko Okuma, Jeffrey M Hord, Ishita Chandel, David Venzke, Mary E Anderson, Ameya S Walimbe, Soumya Joseph, Zeita Gastel, Yuji Hara, Fumiaki Saito, et al.was characterized previously and used at 1:100 (Ervasti and Campbell, 1993). The polyclonal antibody, AF6868 (R&D Systems, Minneapolis, MN, USA; RRID:AB_10891298), was used at a concentration of 1:100 for immunoblotting

(5) Impact of Hypermannosylation on the Structure and Functionality of the ER and the Golgi ComplexBiomedicinesJanuary 6, 2023Franzka P, Schüler SC, Kentache T, Storm R, Bock A, Katona I, Weis J, Buder K, Kaether C, Hübner CA.Biosciences, Heidelberg, Germany), sheep anti-dystroglycan 1:1000 (R&D Systems, Wiesbaden, Germany, AF6868), biotinylated lens culinaris lectin (LCH) 1:300 (Vectorlab, Biozol, Eching, Germany), and biotinylated

(6) Tandem duplication within the DMD gene in Labrador retrievers with a mild clinical phenotypeNeuromuscular DisordersOctober 1, 2022G. Diane Shelton, Katie M. Minor, Natassia M. Vieira, Louis M. Kunkel, Steven G. Friedenberg, Jonah N. Cullen, Ling T. Guo, Mayana Zatz, James R. Mickelsonfollowing blocking buffer diluted antibodies: human α and β dystroglycan (1:1000, R&D Systems Cat # AF6868) and α-tubulin (1:1000, Sigma Cat # T6074). After washing in T-BST buffer, the membrane was incubated

(7) Cancer Malignancy Is Correlated with Upregulation of PCYT2-Mediated Glycerol Phosphate Modification of α-DystroglycanInternational journal of molecular sciencesJune 15, 2022Umezawa F, Natsume M, Fukusada S, Nakajima K, Yamasaki F, Kawashima H, Kuo CW, Khoo KH, Shimura T, Yagi H, et al.anti-α-dystroglycan (IIH6C4) mouse IgM mAb (Millipore, Billerica, MA, USA); anti-human dystroglycan (AF6868) sheep IgG polyclonal antibody (pAb) (R&D systems, Minneapolis, MN, USA); anti-PCYT2 (14827) rabbit

(8) Large1 gene transfer in older myd mice with severe muscular dystrophy restores muscle function and greatly improves survivalScience AdvancesMay 27, 2022Takahiro Yonekawa, Adam J. Rauckhorst, Sara El-Hattab, Marco A. Cuellar, David Venzke, Mary E. Anderson, Hidehiko Okuma, Alvin D. Pewa, Eric B. Taylor, Kevin P. Campbellmembranes were incubated with a sheep polyclonal antibody to human DG (1:100 dilution; R&D Systems, AF6868) and a mouse monoclonal antibody to a glycoepitope on the sugar chain of α-DG (IIH6; 1:100 dilution)

(9) Muscular Dystrophy-Dystroglycanopathy in a Family of Labrador Retrievers with a LARGE 1 MutationNeuromuscular DisordersNovember 1, 2021G. Diane Shelton, Katie M. Minor, Ling T. Guo, Steven G. Friedenberg, Jonah N. Cullen, Jeffrey M. Hord, David Venzke, Mary E. Anderson, Megan Devereaux, Sally J. Prouty, et al.matriglycan (IIH6) was characterized previously [22,30] and used at 1:100. The polyclonal antibody, AF6868 (R&D Systems, Minneapolis, MN), was used at a concentration of 1:100 for the detection of core αDG and

(10) Micro-laminin gene therapy can function as an inhibitor of muscle disease in the dy^W mouse model of MDC1AMolecular therapy. Methods & clinical developmentJune 11, 2021Packer D, Martin PT.for immunoblotting. The antibodies against HB-EGF (AF-259), laminin-α4 (AF3837), and α/β-dystroglycan (AF6868) were obtained from R&D Systems (Minneapolis, MN, USA). The antibody against laminin-α2 G5 (2D4) was

(11) NAD+ enhances ribitol and ribose rescue of α-dystroglycan functional glycosylation in human FKRP-mutant myotubeseLifeJanuary 29, 2021Ortiz-Cordero C, Magli A, Dhoke NR, Kuebler T, Selvaraj S, Oliveira NA, Zhou H, Sham YY, Bang AG, Perlingeiro RC.RRID:AB_2617216",Dilution 1:50 (IF) Antibody,Anti-human dystroglycan (sheep polyclonal),R and D Systems,"Cat# AF6868, RRID:AB_10891298",Dilution 1:1000 (WB) Antibody,Anti-MHC (mouse monoclonal),DSHB,"Cat# MF20, RRID:

(12) Short-term treatment of golden retriever muscular dystrophy (GRMD) dogs with rAAVrh74.MHCK7.GALGT2 induces muscle glycosylation and utrophin expression but has no significant effect on muscle strengthPloS oneJanuary 1, 2021Martin PT, Zygmunt DA, Ashbrook A, Hamilton S, Packer D, Birch SM, Bettis AK, Balog-Alvarez CJ, Guo LJ, Nghiem PP, et al.

(13) POMK regulates dystroglycan function via LARGE1-mediated elongation of matriglycaneLifeSeptember 25, 2020Ameya S Walimbe et al.was characterized previously and used at 1:100 (Ervasti and Campbell, 1991). The polyclonal antibody, AF6868 (R and D Systems, Minneapolis, MN; RRID:AB_10891298), was used at a concentration of 1:200 for immunoblotting

(14) Ribitol enhances matriglycan of α-dystroglycan in breast cancer cells without affecting cell growthScientific reportsMarch 18, 2020Lu PJ, Tucker JD, Branch EK, Guo F, Blaeser AR, Lu QL.primary antibody IIH6C4 at 1:1000 dilution in 3% nonfat dry milk PBS. A sheep anti-α-DG core antibody, AF6868 (R&D Systems, Minneapolis, MN), was used at 1:1000 dilution. Rabbit polyclonal antibody to actin (A2066

(15) biAb Mediated Restoration of the Linkage between Dystroglycan and Laminin-211 as a Therapeutic Approach for α-DystroglycanopathiesMolecular therapy : the journal of the American Society of Gene TherapyFebruary 5, 2020Gumlaw N, Sevigny LM, Zhao H, Luo Z, Bangari DS, Masterjohn E, Chen Y, McDonald B, Magnay M, Travaline T, et al.centrifuged (5,000 rpm for 5 min). The supernatant was assessed by western blot using the polyclonal AF6868 DAG1 antibody (R&D Systems). Systemic Delivery of biAb into LARGEmyd-3J Mice and Analysis of Muscle

(16) A new patient-derived iPSC model for dystroglycanopathies validates a compound that increases glycosylation of α-dystroglycanEMBO reportsNovember 5, 2019Kim J, Lana B, Torelli S, Ryan D, Catapano F, Ala P, Luft C, Stevens E, Konstantinidis E, Louzada S, et al.β‐dystroglycan (1:100; Leica Biosystems, NCL‐b‐DG), and dystroglycan core protein (1:200; R&D Systems, AF6868), followed by washing with TBST and incubation with horseradish peroxidase (HRP)‐conjugated anti‐mouse

(17) Clinical, genetic, and pathologic characterization of FKRP Mexican founder mutation c.1387A>GNeurology GeneticsApril 1, 2019Angela J. Lee et al.Antibodies used for blotting included IIH6 (gift from Kevin P. Campbell, The University of Iowa) and AF6868 (R&D Systems, Minneapolis, MN). Blots were imaged on an Odyssey infrared ﬂuorescence imaging system

(18) LARGE expression in different types of muscular dystrophies other than dystroglycanopathyBMC NeurologyDecember 15, 2018Burcu Balci-Hayta, Beril Talim, Gulsev Kale, Pervin Dincerglycosylated form of αDG, Millipore), 1/50 for sheep anti-α-Dystroglycan core antibody (R&D Systems: AF6868), 1/100 for mouse anti-Spectrin (NCL-SPEC1 Clone: RBC2/3D5, Novocastra), 1:20 for anti-Beta-Dystroglycan

(19) Ribitol restores functionally glycosylated α-dystroglycan and improves muscle function in dystrophic FKRP-mutant miceNature communicationsAugust 27, 2018Cataldi MP, Lu P, Blaeser A, Lu QL.temperature and then incubated with the following primary antibodies overnight at 4 °C: IIH6C4 (1:2000), AF6868 (R&D Systems, AF6868, 1:1000), and α-actin (Sigma, SAB4502543, 1:1000). Appropriate horseradish peroxidase

(20) TIM-1 Mediates Dystroglycan-Independent Entry of Lassa VirusJournal of VirologyAugust 15, 2018Rachel B. Brouillette et al.used in cell surface staining assays were obtained from R&D Systems: sheep anti-hDG (catalog number AF6868), goat anti-hAxl (catalog number AF154), goat anti-hTIM-1 (catalog number AF1750), goat anti-hMer (catalog

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Dystroglycan Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

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Dystroglycan Antibody from R&D Systems, a Bio-Techne Brand

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