Fig 2: Expression of markers of nociceptors, proprioceptors, and mechanoreceptors subtypes in FRDA iPSC-derived sensory neuronal cultures. Differentiated cultures show cluster of neurons expressing NF200 (A). Neurons co-expressing both PRPH (B, D) and PV (C, D) and PRPH (E, G) and SPP1 (F, G) were found. Expression of FAM19A1 (H) and STT (I) was observed. Group of neurons co-expressing PLXNC1 (J, red) and CGRP (K, green). Cell positive for CALB1 (L) was detected. Co-expression of NECAB2 (M, P, green) and TRKB (N, P) was also observed in differentiated cultures. Cells positive for FAM19A1 (Q, T) and TRKA (R, T). C-LTMRs neuron positive both for PRPH (U, X, red, arrowed) and TH (V, X, green, arrowed). Nuclei are shown in blue (DAPI). Scale bars = (A, T, J, K) 20 µm, (D, G, H, I, L, P) 10 µm, (X) 50 µm