Fig 2: Comparison of parameters between the DVT group (n = 48) and the non-DVT group (n = 48), including the (A) TFPI initial anticoagulant time ratio (P = .022), (B) TFPI whole anticoagulant time ratio (P = .048), (C) TFPI anticoagulant rate (P = .034), (D) free-TFPI antigen (P = .035), (E) Prothrombin time (P = .030), and (F) D-dimer (P = .039). * P < .05, and the difference is statistically significant.

Fig 3: Activation of β-catenin is involved in the effect of TFPI on the expression of CXCR7 and the CXCL12-mediated TEM of CLL cells. (A) Western blotting and (B) quantification of the active β-catenin expression, relative to β-actin, after treating the CLL cells from patients with 200 ng/mL rTFPI for 0, 15, 60, 180 and 360 min. n = 3 individuals; *P < 0.05 (C) Quantitative PCR was used to detect the mRNA expression of CXCR7, relative to TBP, after CLL cells were pre-treated with IWP4 (5 µM) for 30 min prior to 24 h treatment of 200 ng/mL rTFPI, with DMSO as a control. n = 4 individuals; *P < 0.05, **P < 0.01. (D) Western blotting and (E) quantification of CXCR7 expression, relative to β-actin, after CLL cells were pre-treated with 5 µM IWP4 for 30 min prior to 24 h treatment of 200 ng/mL rTFPI, with DMSO as a control. n = 3 individuals; *P < 0.05. (F) CLL cells were pre-treated with 5 µM IWP4 for 30 min prior to 24 h treatment of 200 ng/mL rTFPI, with DMSO as a control. Then CLL cells were washed and applied to TEM assay. TEM results were presented as we described in Fig. 2A. n = 10 individuals; **P < 0.01.

Fig 4: Mycolactone reduces the expression of tissue factor pathway inhibitor in endothelial cells.A. HDMECs exposed to 10 ng/mL of mycolactone (MYC) for the indicated times, 0.02% DMSO or untreated (Ctrl) were lysed and subjected to immunoblotting. TFPI immunoblot intensity was normalised according to GAPDH and untreated controls. Data from 3 independent experiments are presented (mean ± SEM). B. Supernatant was collected from cells treated as above for 24 hours, cell debris removed and TFPI was quantified by ELISA. Values represent the mean of three independent experiments ± SEM. ***, P < 0.001; ****, P < 0.0001.

Fig 5: The curve of TFPI anticoagulant activity.