Fig 2: Effects of CHB-II-F on mRNA expression of NPY, AgRP, POMC, CART, OX1R, Ob-R,., and GHSR in the hypothalamus of the transplanted H22 hepatocellular carcinoma model mice receiving chemotherapy (x¯ ± s, n = 6). (A) NPY mRNA expression in different groups. (B) CART mRNA expression in different groups. (C) GHSR mRNA expression in different groups. (D) Ob-R mRNA expression in different groups. (E) POMC mRNA expression in different groups. (F) OX1R mRNA expression in different groups. (G) AgRP mRNA expression in different groups. (H) Gel electrophotogram of target gene NPY, AgRP, POMC, CART, OX1R, Ob-R, GHSR, and ß-actin amplification products of real-time fluorescent quantitation polymerase chain reaction (RTFQ-PCR). 1: AgRP; 2: NPY; 3: POMC; 4: CART; 5: OX1R; 6: Ob-R; 7: GHSR. (I) Gel electrophotogram of total RNA of tumor tissue. Randomly select three samples from each group and verify their integrity. 1–3: saline-treated controls (model); 4–6: 5-FU treated controls (0.02 g/kg); 7–9: YZXJC (0.78 g/kg); 10–12: CHB-II-F(H) (65 g/kg); 13–15: CHB-II-F(M) (32.5 g/kg); 16–18: CHB-II-F(L) (16.25 g/kg). Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001 compared with the model (saline) group. # p < 0.05, ## p < 0.01 compared with the 5-FU group. ? p < 0.05 compared with the YZXJC group.

Fig 3: Effects of CHB-II-F on protein expression of NPY, AgRP, POMC, CART, OX1R, Ob-R, and GHSR in the hypothalamus of the transplanted H22 hepatocellular carcinoma model mice receiving chemotherapy detected by immunohistochemistry (x¯ ± s, n = 6). (A) Immunohistochemical pictures of NPY, AgRP, POMC, CART, OX1R, Ob-R, and GHSR protein expression in hypothalamus (× 400, scale bar = 200 µm). (B) NPY IOD values in different groups. (C) AgRP IOD values in different groups. (D) POMC IOD values in different groups. (E) CART IOD values in different groups. (F) OX1R IOD values in different groups. (G) Ob-R IOD values in different groups. (H) GHSR IOD values in different groups. Statistical analysis: *p < 0.05, **p < 0.01, ***p < 0.001 compared with the model (saline) group. # p < 0.05, ## p < 0.01 compared with the 5-FU group. ? p < 0.05 compared with the YZXJC group.