Fig 1: Frequency of B Cell Subsets in Peripheral Blood of HIV-1 Env-Immunized Neonatal and Adult MacaquesFrequency of eight B cell subsets, including naive, IgM+ memory, IgG+ memory, IgM- IgG- memory, IgM+ plasmablasts, IgM- plasmablasts, marginal zone-like (in log scale), and a rare population of undefined B cells (Query 1; Ki67+CD1c+CD27+ IgM2+) among CD20+CD3- CD14- CD16- lymphocytes from CH505-SOSIP-immunized neonatal (blue) and adult (red) macaques in studies 2 and 3 measured by flow cytometry 2 weeks after the first, third, and sixth immunizations. Horizontal bars represent the group mean. nparLD was performed on SOSIP-immunized animals combined in studies 2 and 3 (p = 0.007 for naive, p = 0.69 for IgM+ memory, p = 0.0005 for IgG+ memory, p = 0.0002 for IgM- IgG- memory, p = 0.027 for IgM+ plasmablasts, p = 0.04 for IgM plasmablasts, p = 0.00009 for marginal zone-like, and p = 0.0007 for Query 1). The scales of the y axis are different on each graph.