Fig 1: IKKe knockout inhibited the NF-?B signaling pathway in Dox-induced DCM. (A). Representative western blots showing total protein and the phosphorylation levels of I?Ba, RelB, p65, and p100 in the NF-?B pathway in heart tissues of WT and IKKe-KO mice injected with Dox (n = 6 mice per experiments). (B). Quantitative analysis of western blotting of proteins related to the NF-?B pathway (n = 6 mice per experimental group; # p/*p < 0.05 vs. Saline or WT DOX). (C). Representative western blots showing the nuclear and cytoplasmic protein levels of p65 in the NF-?B pathway in heart tissues from WT and IKKe-KO mice injected with Dox (n = 6 independent experiments). (D). Relative quantitative analysis of nuclear translocation of p65 (n = 6 mice per experimental group; # p/*p < 0.05 vs. Saline or WT DOX).
Fig 2: IKKe deficiency inhibits the Ang II-mediated MEK1/2-ERK1/2 and P38 signaling pathways. (a, b) Representative western blots showing the phosphorylation and total protein levels of the MAPK pathway proteins MEK1/2, ERK1/2, JNK, and p38 and the NF-?B pathway proteins I?Ba, p65, RelB, and p100 in the heart tissue of WT and IKKe-KO mice that were infused with Ang II for 8 weeks (n = 6 independent experiments). (c) Relative quantitative analyses of western blot analysis of the MAPK pathway (n = 6 mice per experimental group; *P < 0.05 vs. saline or WT Ang II). (d) Relative quantitative analyses of western blot analysis of the NF-?B pathway (n = 6 mice per experimental group; #P < 0.05 vs. saline).
Supplier Page from Abcam for Anti-NFkB p100/NFKB2 antibody [EPR4686]