Fig 1: Expression of distinct alveolar epithelial cell markers in AXiAECs cultured on AXLung-on-chip. (A) Timeline and schematic of the AXiAECs cultured on the AX12. (B) Representative immunofluorescent staining for AXiAECs fixed after 7 days cultured in AX12 (D7), probed for epithelial: EPCAM (red), MUC1 (red); AT1-like: HTI-56 (green), HOPX (red). and AT2-like markers: SP-C (green), ABCA3 (red). Nuclei were stained with DAPI (blue). Scale bar is provided with each image. (C) Relative gene expression of distinct epithelial and (D) alveolar genes in A549 cells (N = 1; n = 3) and AXiAECs cultured on AXLung-on-chip (N = 2; n = 4) for 7 days. (E) AXiAECs stained for ACE2 (cyan) and TMPRSS2 (green). (F) qPCR results show gene expression of SARS-CoV-2 host factors in AXiAECs (N = 2; n = 4) after 7 days of culture in AX12. Data are shown as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001.
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