Fig 1: Effects of MALAT1/miR-204/APOL1 axis on HK-2 cell proliferation and apoptosis under hypoxic conditions. (A) Expression of APOL1 after 12-h hypoxia treatment in HK-2 cells. (B) Transfection efficacy of APOL1 in hypoxia-induced HE-2 cells. (C) CCK-8 results. (D) Cell viability evaluated by colony formation analysis. (E) Cell apoptosis determined by flow cytometry analysis. (F) TUNEL staining assay
Fig 2: Effects of MALAT1/miR-204/APOL1 axis on inflammation in hypoxia-treated HK-2 cells
Fig 3: miR-204 targeted APOL1 to abrogate the inhibitory effects of MALAT1 knockdown by activation of the NF-?B pathway in HK-2 cells. (A) The potential binding sequences between APOL1 and miR-204. (B) Luciferase reporter assay. (C) RNA pull-down assay. (D) Protein expressions of APOL1, p65, and p65 in HK-2 cells after hypoxia. (E) APOL1, p65, and p-p65 levels after co-transfection with LV-MALAT1, inhibitor-NC, and miR-204 inhibitor in HK-2 cells exposed to hypoxia
Supplier Page from Abcam for Anti-Apolipoprotein L1/APOL1 antibody [EPR2907(2)]