Fig 1: Proteomic analyses of UQCR10 and CYC1-containing protein associations in ?4-CYB cells. See also Fig EV3 SDS–PAGE, Western blot, and immunodetection, with the indicated specific antibodies, of ?4-CYB and WT cells expressing HA-tagged versions of UQCRQ and UQCR10 and of cells transduced with the lentiviral expression vector without any cDNA insert (Empty).BNGE, Western blot, and immunodetection, with an anti-HA tag antibody, of samples from the same cell lines as in (A) solubilized either with digitonin or DDM.BNGE, Western blot, and immunodetection, with the monoclonal (M) anti-UQCRQ antibody (Abcam ab110255), of non-transduced ?4-CYB and WT cells. The mitoplast samples were solubilized with DDM (See also Fig EV1).Scatter plot generated from the analysis of the logarithmic heavy (H)-to-light (L) ratios in the x-axis and the reverse in the y-axis, in the two reciprocal labeling SILAC experiments (1 and 2) and anti-HA immunopurification of ?4-CYB and WT cells expressing UQCR10HA.Complexome profiles, generated as in Fig 3, for the proteins found specifically enriched in ?4-CYB UQCR10HA, according to the SILAC immunopurification experiments shown in (D). The represented values are the mean ± SEM of the two reciprocal labeling experiments.SDS–PAGE, Western blot, and immunodetection, with the indicated specific antibodies, of ?4-CYB and WT cells expressing an HA-tagged version of CYC1 and of cells transduced with the lentiviral expression vector without any cDNA insert (Empty).BNGE, Western blot, and immunodetection, with an anti-HA tag antibody, of samples from the same cell lines as in (F) solubilized either with digitonin.Scatter plot generated from the analysis of the logarithmic heavy (H)-to-light (L) ratios in the x-axis and the reverse in the y-axis, in the two reciprocal labeling SILAC experiments (A and B) of anti-HA immunopurification of ?4-CYB and WT cells expressing CYC1HA. Source data are available online for this figure.