Fig 1: Mitochondrial localization of SFXN2. mCherry-SFXN2 (a) and SFXN2-mCherry (b) colocalized with MitoTracker. Bar = 5 µm. mCherry-SFXN2 (c) and SFXN2-mCherry (d) colocalized with MitoTracker and endogenous Tomm20. Arrows indicate mCherry-SFXN2 or SFXN2-mCherry, while arrowheads indicate Tomm20. The fluorescence intensities along the dashed lines are shown as line profile graphs. Bars = 1 µm. e Mitochondria were isolated from HEK293 cells transfected with SFXN2-mCherry and then digested with trypsin. SFXN2, Mitofusin1, and Timm50 were detected by Western blotting. The arrow indicates bands corresponding to SFXN2-mCherry. f Quantification of SFXN2-mCherry, Mitofusin1, and Timm50. n = 3–4 each. *p < 0.05, **p < 0.01, ****p < 0.0001 by a two-way ANOVA