Fig 1: Overall survival curves of hub genes in diffuse-type GC. The x-axis represents overall survival time and the y-axis represents survival rate. Patients with expression above the median are indicated in red line, and patients with expressions below the median in black line (A-C) Survival analysis evaluating the effects (A) AGT, (B) CXCL12 and (C) ADRB2 expression on survival in the GSE62254 dataset. (D-F) Survival analysis evaluating the effects of (D) AGT, (E) CXCL12 and (F) ADRB2 expression on survival in the GSE15459 dataset. GC, gastric cancer; AGT, angiotensinogen; CXCL12, C-X-C motif chemokine ligand 12; ADRB2, adrenoceptor ß2, HR, hazard ratio.
Fig 2: Immunohistochemical detection of AGT, CXCL12 and ADRB2 expression in intestinal and diffuse-type GC tissue samples. Magnification, ×400. Scale bar, 50 µm. GC, gastric cancer; AGT, angiotensinogen; CXCL12, C-X-C motif chemokine ligand 12; ADRB2, adrenoceptor ß2.
Fig 3: Characterization of ONH astrocytes from explants of the rat LC plants. (A) Cells grew out of the explants of the LC within 1 week and reached confluence at about 4 weeks. ONH astrocytes had several processes with star shape morphology. LC cells appeared as flat, broad shaped cells. (B) ONH astrocytes showed positive staining for GFAP and LN. (C) LC cells showed negative staining for GFAP and positive staining for LN. (D,E) ONH astrocytes exhibited positive staining for ET-1, NOS1, NOS2, rennin, AGT, Ang, and ACE1, with negative staining for NOS3. ONH, Optic nerve head; LC, Lamina cribrosa; ET-1, Endothelin 1; GFAP, Glial fibrillar acid protein; LN, Laminin; ET-1, Endothelin 1; NOS, Nitric oxide synthase; AGT, Angiotensinogen; Ang, Angiotensin; ACE1, Angiotensin converting enzyme 1.
Fig 4: Systematic activation of RAS promoted the local expression of ACE in SHR nucleus pulposus tissue in vivo.(a, b) The blood pressure was measured with a noninvasive Tail-Cuff System Western (ALC-NIBP, Shanghai Alcott Biotech) at 3 M, 6 M, and 12 M, respectively, according to previous study [34]. (c) Western blot results for the expression of ACE and AT1 in the rat NP tissue at the age of 6 and 12 months, respectively (n = 3). (d) The results of western blot were quantified (n = 3). (e) Immunofluorescence results for the expression of AGT in the rat NP tissue at the age of 12 months (n = 3). (f) The results of immunofluorescence were quantified (n = 3). SBP: systolic blood pressure; DBP: diastolic blood pressure. p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Fig 5: Immunofluorescent staining of NOS and RAS components in rat LC tissues. (A) Longitudinal section of LC tissue showed positive staining of Ang, renin, AGT, NOS1, NOS3, and GC. (B) Horizontal section of LC tissue showed positive staining of Ang, renin, AGT, NOS1, NOS3, and GC. LC, Lamina cribrosa; Ang, Angiotensin; AGT, Angiotensinogen; NOS, Nitric oxide synthase; GC, Guanylate cyclase.
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