Fig 1: Fhit interacts with Fdxr, HSP10, and HSP60.a GST pull-down experiment using a protein lysate from H1299D1 cells transfected with HA-FDXR which was incubated with GST and GST-Fhit protein bound to glutathione agarose resin, and protein complexes eluted and separated on acrylamide gel for detection with antisera against HA, HSP60, and Fhit. b Lysates were prepared from DSP-cross-linked H1299D1 cells (Pon A induced for Fhit expression) or from HCT116 cells (expressing endogenous Fhit) and used in IP experiments with the indicated antisera. Complexes were separated on acrylamide gel and proteins were detected with HSP60, Fhit, or HSP10 antisera. c The association of Fhit and Fdxr in HCT116 cells using the Duolink in situ proximity ligation assay (PLA). PLA signals are shown in red and nuclei in blue. PLA signals for Fhit/Fdxr are mostly confined in the cytoplasm. Negative controls with the Fdxr or Fhit antibody alone show no PLA signal. Scale bar represents 10 µm