Fig 1: S100A11 enhances the membrane translocation of ANXA1 through a mechanism involving PEX14 and actively inhibits the nuclear translocation of ANXA1.a, b Co-IP showing the binding of S100A11 (a) or ANXA1 (b) to PEX14. c–e Western blot (c) showing the subcellular location of S100A11 and ANXA1 in N2a cells treated with His-PEX14 or shPEX14 and the quantitative analysis of subcellular levels of S100A11 (d) and ANXA1 (e). f–h Western blot (f) showing the subcellular localization of S100A11 and ANXA1 in N2a cells transfected with His-PEX14 or shPEX14 after OGD/R and the quantitative analysis of subcellular levels of S100A11 (g) and ANXA1 (h). Data are reported as the means ± S.E.M. from three independent experiments. i–k Western blot showing the subcellular localization of ANXA1 in N2a cells transfected with shS100A11 or S100A11 and/or PEX14 or shPEX14 after OGD/R, and quantitative analysis of subcellular levels of ANXA1. *P < 0.05; **P < 0.01; ***P < 0.001; n.s.: the difference between the two groups was not significant. Data are reported as the means ± S.E.M. from three independent experiments
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