Fig 1: Double immunofluorescence staining for pSmad2/3L-Thr (red; arrowheads) with (A–C) ß-catenin (green), (E–G) Ki67 (green), (I–K) CDK4 (green), and (M–O) Bmi1 (green) in the micro-neoplastic lesions of the AOM/DSS mice. DAPI (blue) was used for nuclear staining. (D,H,L,P) The same sections were stained with hematoxylin and eosin after immunofluorescence staining and observed via light microscopy. Original magnification, ×200 (A–P). Scale bars: 300 µm (A–P).
Fig 2: Double immunofluorescence staining for pSmad2/3L-Thr (red; arrowheads) with (A–C) ß-catenin (green), (E–G) Ki67 (green), (I–K) CDK4 (green), and (M–O) Bmi1 (green) in the uni-cryptal neoplastic lesions of the AOM/DSS mice. DAPI (blue) was used for nuclear staining. (D,H,L,P) The same sections were stained with hematoxylin and eosin after immunofluorescence staining and observed via light microscopy. Original magnification, ×200 (A–P). Scale bars: 100 µm (A–P).
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