Fig 1: J774A.1 cells were primed with 100 ng/ml LPS and inhibited with 1 µM MCC950 or 200 µM Glyburide then stimulated with 5 mM ATP. (a) The phosphorylation state of NEK7 was analysed using Phos-tag SDS-PAGE. These results are representative of three independent experiments. (b) ELISA analysis of IL-1β in the culture supernatant of J774A.1 cells treated as in (a). Data are expressed as the mean ± sem of three independent experiments carried out in duplicates. ***P < 0.001 (one-way ANOVA with Tukey’s post-hoc test).