Fig 1: CERS6 expression in LNM35 cells regulated by CEBP? and YBX1. Western blot analysis results showing CERS6 protein level after silencing of (A) CEBP?, (B) CEBP?, (C) NFYA and NFYB, and (D) YBX1. CEBPG expression levels were measured using an RT-PCR assay (Figure S3A), as no reliable antibody could be found. Luciferase reporter analysis results with (E) siCTRL, (F) siCEBP?#1 and (G) siYBX1#1. Values are shown as the mean ± SD (n = 3). *P < .05. (H) ChIP assays performed with anti-HA and anti-YBX1 antibodies using LNM35 cells. Values are shown as the mean ± SD (n = 4). *P < .05
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