Fig 1: Effect of NKT of lipid marker enzymes in the plasma and liver: (A,B) activity of 3-hydroxy-3-methylglutaryl coenzyme-A reductase (HMG-CoA) reductase in the plasma and liver. Each column is the mean ± SEM for eight rats in each group; * p < 0.05 as compared to the normal control (Group-I), ** p < 0.05 as compared to the ISO control (Group-III) (DMRT). In group III, the lower ratio of HMG-CoA/mevalonate indicates higher enzyme activity and, in Group IV, a higher ratio of HMG-CoA/mevalonate indicates lower enzyme activity; (C,D) activity of lecithin cholesterol acyltransferase (LCAT) in the plasma and liver. Each column is the mean ± SEM for eight rats in each group; * p < 0.05 as compared to the normal control (Group-I), ** p < 0.05 as compared to the ISO control (Group-III) (DMRT). * Units: μM of cholesterol esterified/h/mL for plasma LCAT and μM of cholesterol esterified/h/mg protein for liver; (E,F) Western blotting analysis of HMG-CoA-R and LCAT in the liver and the densitometric analysis. Columns not sharing a common symbol (*, **) differ significantly from each other (* p < 0.05 vs. normal control, ** p < 0.05 vs. ISO control).