Fig 1: NPR1, DCAF4L2 and TSSK6 expression is increased in glioma, and elevated NPR1 or TSSK6 expression is a prognostic indicator of poor survival in patients with classical glioma. A, NPR1, TSSK6 and DCAF4L2 mRNA expression in multiple tumour tissues and normal tissues based on RNA-Seq data from the TCGA. Log2-normalized read count (TPM) is shown. TPM, transcripts per kilobase of exon model per million mapped reads. B, mRNA expression of NPR1, DCAF4L2 and TSSK6 was analysed in glioma tissues from the TCGA data sets. C, Kaplan–Meier survival curve analysis indicated that patients with NPR1 and TSSK6 overexpression had a significantly shorter overall survival in the classical subtype of glioma. D, E, NPR1 protein expression levels were elevated in primary glioma patient samples. F, Immunohistochemical staining of NPR1 in glioma tissue