Fig 1: Talin-1 promotes HCC growth and metastasisTalin-1 is more highly expressed in MHCC-97L cells compared with LO2 cells in terms of (A) mRNA levels and (B) protein amounts. Talin-1 expression is lower in the sh-Talin-1 group than the NC and sh-mock groups in (C) mRNA levels and (D) protein amounts. (E) Cell proliferation was assessed by counting. Markedly fewer cells were found in the sh-Talin-1 group compared with NC and sh-mock groups at 24 h, 48 h or 72 h; (F, G), HCC cell migration ability was assessed by transwell migration assay. Migration was significantly reduced in the sh-Talin-1 group compared with the NC and sh-mock groups. *P < 0.05 **P < 0.01, and ***P < 0.001. The data are presented as the mean SEM.
Fig 2: Talin-1 regulates cell adhesion in HCC(A) The heat map of genes changed by two fold or more in cell adhesion after Talin-1 silencing in HCC. The signal value of the genes is higher in yellow box than in blue ones; (B–F) Expression of selected genes in cell adhesion measured by qRT-PCR. (B) MUC1, (C) WISP1, (D) SLAMF7, (E) VTN and (F) FGB were down-regulated in the sh-Talin-1 group compared with the NC and sh-mock groups. *P < 0.05 **P < 0.01, and ***P < 0.001. The data are presented as the mean SEM.
Fig 3: Talin-1 promotes ion transport and membrane depolarization in HCC(A, B) Heat maps of genes changed by two fold or more after Talin-1 knockdown involved in (A) ion transport and (B) membrane depolarization. The signal value of the genes is higher in yellow box than in blue ones. (C–G) Expression of selected genes involved in ion transport and membrane depolarization are measured by qRT-PCR. (C) CAV3, (H) AQP1, (E) PTGS2, (F) CA9 and (G) CLIC3 were down-regulated while (D) KCNE3 was up-regulated in the sh-Talin-1 group compared with the NC and sh-smock groups. *P < 0.05 **P < 0.01, and ***P < 0.001. The data are presented as the mean ± SEM.
Fig 4: Talin-1 promotes cell growth in HCC(A) The heat map of cell growth-related genes changed by two fold or more after Talin-1 silencing in HCC. The signal value of the genes is higher in the yellow box than in blue ones; (B–E), Expression of selected cell growth-related genes measured by qRT-PCR. (B) EDN1, (C) IGFBP5, (D) CYR61 and (E) IGFBP3 were downregulated in the sh-Talin-1 group compared with the NC and sh-mock groups. *P < 0.05 **P < 0.01, and ***P < 0.001. The data are presented as the mean ± SEM.
Fig 5: Interaction network of Talin-1 in HCC(A) putative physical and regulatory interactions between Talin-1 and candidate transcriptional regulators, and between the later and their target genes in HCC; (B) pathways regulated by Talin-1 and candidate transcriptional regulators in HCC. Talin-1 is in red; the 11 selected genes are in yellow; the other searched genes are in blue.
Supplier Page from Abcam for Anti-Talin 1 antibody [93E12]