Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] ab109524 from Abcam

Product Specs
ItemAnti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab109524
ClonalityMonoclonal
Quantity10 UL, 40 UL, 100 UL
ApplicationsFlow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections), Western blot
IsotypeIgG
Antibody TypePrimary
CloneEPR5377
NCBI Gene AliasesTOPIIB, top2beta
Conjugate/TagUnconjugated
HostRabbit
Gene NameTOP2B
ReactivityHuman, Mouse, Rat, Recombinant fragment
TargetTopoisomerase II alpha+Topoisomerase II beta

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Website: http://www.abcam.com

(1) GCNA is a histone binding protein required for spermatogonial stem cell maintenanceNucleic Acids ResearchJune 9, 2023Jonathan Ribeiro, Gerry P CrossanAbcam); anti-Nanog (1:1000, catalog no. 8822, Cell Signaling Technology); anti-TOP2 (1:1000, catalog no. ab109524, Abcam); anti-SYCP3 (1:500, catalog no. ab15093, Abcam), anti-GroEL; anti-histone H1 (1:500, catalog

(2) SPIDR is required for homologous recombination during mammalian meiosisNucleic Acids ResearchMay 8, 2023Tao Huang, Xinyue Wu, Shiyu Wang, Ziyou Bao, Yanling Wan, Ziqi Wang, Mengjing Li, Xiaochen Yu, Yue Lv, Zhaojian Liu, et al.slide. After air drying, oocytes were stained with the Topoisomerase II antibody (1:400 dilution; Abcam #ab109524) to detect chromosomes. Immunohistochemistry The ovaries of E13.5, E15.5, PD1, PD8 and PD15 were immunohistochemical.

(3) Taspase1 Facilitates Topoisomerase IIβ-Mediated DNA Double-Strand Breaks Driving Estrogen-Induced TranscriptionCellsJanuary 18, 2023Oelschläger L, Stahl P, Kaschani F, Stauber RH, Knauer SK, Hensel A.anti-Nucleophosmin1/B23 (sc-47725, Santa Cruz Biotechnology, Heidelberg, Germany), anti-Topoisomerase IIα/β (ab109524, Abcam, Cambridge, UK), anti-Topoisomerase IIα (sc-365071, Santa Cruz Biotechnology, Heidelberg, Germany),

(4) Replication and transcription machinery for ranaviruses: components, correlation, and functional architectureCell & bioscienceJanuary 6, 2022Ke F, Yu XD, Wang ZH, Gui JF, Zhang QY.of host topoisomerase II in ranavirus infection, a commercial anti-topoisomerase II antibody (Abcam, ab109524, immunogen of the antibody was declared as a fraction of the human TopIIβ) was used based on the high

(5) High Antitumor Activity of the Dual Topoisomerase Inhibitor P8-D6 in Breast CancerCancers (Basel)December 21, 2021Inken Flörkemeier, Tamara N. Steinhauer, Nina Hedemann, Jörg Paul Weimer, Christoph Rogmans, Marion T. van Mackelenbergh, Nicolai Maass, Bernd Clement, Dirk O. Bauerschlag1:500 (Santa Cruz Biotechnology, #sc-271285, (Heidelberg, Germany)), anti-Topo II α/β 1:10000 (Abcam#ab109524, (Cambridge, UK)) and anti-HSP 90 1:10000 (Santa Cruz Biotechnology, #sc-13119, (Heidelberg, Germany)).

(6) Newly developed dual topoisomerase inhibitor P8-D6 is highly active in ovarian cancerTherapeutic Advances in Medical OncologyNovember 25, 2021Inken Flörkemeier, Tamara N. Steinhauer, Nina Hedemann, Magnus Ölander, Per Artursson, Bernd Clement, Dirk O. Bauerschlagincubated with primary antibodies (anti-TopoI 1:500 (Santa Cruz#sc-271285), anti-TopoIIα/β 1:10000 (Abcam#ab109524), anti-HSP 90 1:10000 (Santa Cruz#sc-13119)) and HRP-labelled anti-mouse IgG 1:2000 (Santa Cruz#sc-516102)

(7) Loss of sister kinetochore co-orientation and peri-centromeric cohesin protection after meiosis I depends on cleavage of centromeric REC8Developmental cellNovember 22, 2021Ogushi S, Rattani A, Godwin J, Metson J, Schermelleh L, Nasmyth K.Yoshinori Watanabe, University of Tokyo, Japan; 1:100 and 1:100, respectively), anti-TOPOII (Abcam, #ab109524, 1:100), anti-histone H3 tri-methylated at lysine 9 (H3K9me3; Abcam, #ab8898, 1:250-500) and anti-alpha

(8) Green Tea (Camellia sinensis) Extract Increased Topoisomerase IIβ, Improved Antioxidant Defense, and Attenuated Cardiac Remodeling in an Acute Doxorubicin Toxicity ModelOxidative Medicine and Cellular LongevityMay 5, 2021Pamela N. Modesto et al.Technology (Ser473); cs 4060S,1/2000 AKT,Cell Signaling Technology; cs 9272,1/1000 Top2-β,Abcam; ab109524,1/10000 GAPDH,Santa Cruz; sc 32233,1/10000 Concentrations of primary antibodies used for western blot

(9) Deprotection of centromeric cohesin at meiosis II requires APC/C activity but not kinetochore tensionThe EMBO JournalApril 1, 2021Valentina Mengoli, Katarzyna Jonak, Oleksii Lyzak, Mahdi Lamb, Lisa M Lister, Chris Lodge, Julie Rojas, Ievgeniia Zagoriy, Mary Herbert, Wolfgang Zachariaeanti‐centromere antibodies (ACA, Antibodies Inc. 15‐235, 1:50) and rabbit antibodies to topoisomerase II (Abcam ab109524, 1:100) or Sgol2 (a gift from José L. Barbero; Gomez et al, 2007; 1:100). After washing, slides were

(10) MiRNA‐145‐5p expression and prospective molecular mechanisms in the metastasis of prostate cancerIET Systems BiologyFebruary 1, 2021Zhi‐Guang Huang, Yu Sun, Gang Chen, Yi‐Wu Dang, Hui‐Ping Lu, Juan He, Ji‐Wen Cheng, Mao‐Lin He, Sheng‐Hua Liconducted as the manufacturers' protocols guided. Rabbit monoclonal anti‐TOP2A (1:100 diluted; catalog no. ab109524, Abcam, Cambridge, MA, USA) was the first antibody for IHC. The intensity and the percentage of positive

(11) Ubiquitin stimulated reversal of topoisomerase 2 DNA-protein crosslinks by TDP2Nucleic Acids ResearchJune 19, 2020Matthew J Schellenberg, C Denise Appel, Amanda A Riccio, Logan R Butler, Juno M Krahn, Jenna A Liebermann, Felipe Cortés-Ledesma, R Scott Williamsrabbit monoclonal anti-TOP2␣: Abcam (ab52934 lot# GR241638-22), rabbit monoclonal anti-TOP2␤: Abcam (ab109524, lot# GR42963-15), mouse monoclonal anti SUMO2/3: Abcam (ab81371 lot# GR3233360-1), mouse anti-GFP/YFP: Roche

(12) Investigation of Structure-Activity Relationships of Dexrazoxane Analogs Reveals Topoisomerase IIβInteraction as a Prerequisite for Effective Protection against Anthracycline CardiotoxicityJournal of Pharmacology and Experimental TherapeuticsJune 1, 2020Petra Kollárová-Brázdová et al.seconds). Four to eight independent experiments were performed in each group. as that used for NVCMs (ab109524; dilution 1:1000; Abcam) and secondary anti-rabbit antibody (NA934; dilution 1:2000; GE Healthcare,

(13) Distribution of DNA damage in the human sperm nucleus: implications of the architecture of the sperm headAsian Journal of AndrologyJanuary 1, 2020MaríaPaz González-Rojo, Cristina Fernández-Díez, Cristina Fernández-Díez, María Paz Herráezantibody (ab62623, “anti-DNA/RNA damage antibody,” Abcam); 1/20 rabbit monoclonal TOPO IIα + β antibody (ab109524, Abcam); 1/200 rabbit polyclonal Histone H3 antibody (ab1791, Abcam); and 1/200 rabbit polyclonal PRDX6

(14) Dissociation of membrane-chromatin contacts is required for proper chromosome segregation in mitosisMolecular biology of the cellFebruary 15, 2019Champion L, Pawar S, Luithle N, Ungricht R, Kutay U.ab32535), mAb414 (Abcam, ab24609), anti-MAD2 (Bethyl Laboratories, A300-301A), anti-topoisomeraseII (Abcam, ab109524), anti–α-tubulin (Sigma, T5168), and anti–γ-H2AX (Millipore, 05-636-l). The antibody against human SUN1

(15) DNA topoisomerase 1 and 2A function as oncogenes in liver cancer and may be direct targets of nitidine chlorideInternational Journal of OncologyAugust 21, 2018Li-Min Liu, Dan-Dan Xiong, Peng Lin, Hong Yang, Yi-Wu Dang, Gang Chencatalog no. ab109374) and rabbit monoclonal anti-TOP2A + TOP2B antibody (1:100 dilution; catalog no. ab109524) (both from Abcam, Cambridge, MA, USA) were used for the tissue microarrays. Mouse monoclonal anti-TOP1

(16) Cohesin Can Remain Associated with Chromosomes during DNA ReplicationCell ReportsSeptember 19, 2017James D.P. Rhodes, Judith H.I. Haarhuis, Jonathan B. Grimm, Benjamin D. Rowland, Luke D. Lavis, Kim A. NasmythProcedures Antibodies Antibodies included Scc1 (ab154769, Abcam), PCNA (ab29, Abcam), and Topo II alpha and beta (ab109524, Abcam). HaloTag/SNAP-Tag Ligands JF549-SNAPTag, JF646-SNAPTag, JF549-HaloTag, and JF646-HaloTag were

(17) Characterization of muscle ankyrin repeat proteins in human skeletal muscleAmerican journal of physiology. Cell physiologySeptember 1, 2017Wette SG, Smith HK, Lamb GD, Murphy RM.Bank A4.840/Development Studies Hybridoma Bank 6H1/Development Studies Hybridoma Bank A010-20/Badrilla Ab109524/Abcam PIE31430/Thermo Fisher Scientific PIE31460/Thermo Fisher Scientific Sc2064/Santa Cruz Dilution Mouse

(18) Reduction in promotor methylation utilizing EGCG (epigallocatechin-3-gallate) restores RXRα expression in human colon cancer cellsOncotargetJune 7, 2016Jay Morris, Vondina R. Moseley, April B. Cabang, Katie Coleman, Wei Wei, Elizabeth Garrett-Mayer, Michael J. Wargovich07-1651 1:500, Millipore, Billerica, MA, USA, anti-Cyclin D1 [SP4] ab16663 1:200, anti-TOPO IIβ [EPR5377] ab109524 1:1000, Abcam, Cambridge, MA, USA) were incubated overnight followed by the appropriate secondary for

(19) CRL4-DCAF1 ubiquitin E3 ligase directs protein phosphatase 2A degradation to control oocyte meiotic maturationNature communicationsAugust 18, 2015Yu C, Ji SY, Sha QQ, Sun QY, Fan HY.

(20) Transcription-dependent dynamic supercoiling is a short-range genomic forceNature Structural & Molecular BiologyMarch 1, 2013Fedor Kouzine, Ashutosh Gupta, Laura Baranello, Damian Wojtowicz, Khadija Ben-Aissa, Juhong Liu, Teresa M. Przytycka, David Levens

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Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] from Abcam

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Anti-Topoisomerase II alpha + Topoisomerase II beta/TOP2B antibody [EPR5377] from Abcam

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