Fig 1: Overexpression of ENO1 enhanced the expression of apoptosis and glucose metabolic-related proteinsThe expression of apoptosis proteins in cells overexpressing ENO1 was determined using Western blotting as described in Materials and Method section (A). The expression of gluconeogenesis-related protein in cells overexpressing ENO1 was presented in (B).
Fig 2: Overexpression of ENO1 reversed the effect of GRN A on cell migration and invasion(A) and (B) indicated the migration and invasion in ENO1 overexpressing cells as analyzed using Transwell experiment, while (C), and (D) represented the quantitative results of (A) and (B). (E) indicated the levels of ENO1 in transfected with the ENO1 construct.
Fig 3: Interaction of GRN A with ENO1(A) SDS–PAGE/His-pull down assay. SDS–PAGE/His-pull down assay was performed as described in the Materials and Method section. The prey protein band was indicated by an arrow. (B)Spectra of the prey peptide as analyzed by LC-MS/MS, which were identified as ENO1. (C) Amino acid sequence of ENO1. The red font represented the sequence analyzed by MS. (D) Western blotting analysis. The gel was immunoblotted using anti-ENO1 monoclonal antibody. (E) SPR analysis. The interaction of GRN A and ENO1 was analyzed using BIACORE T200.
Supplier Page from Abcam for Anti-ENO1 antibody [2G2AG11BF8]