Fig 1: Comparison of Shh, Gli-1, PTC1, PTC2, Nestin and Smo mRNA expression among the seven groups. Notes: (a) relative Shh mRNA expression among the seven groups; (b) relative Gli-1 mRNA expression among the seven groups; (c) relative Smo mRNA expression among the seven groups; (d) relative Nestin mRNA expression among the seven groups; (e) relative PTC1 mRNA expression among the seven groups; (f) relative PTC2 mRNA expression among the seven groups; Shh, sonic hedgehog; Gli-1, glioma-associated oncogene homolog 1; PTC1, Patched 1; PTC2, Patched 2; SCI, spinal cord injury; NC, negative control; *P<0.05 compared with the normal group; #P<0.05 compared with the SCI group; &P<0.05 compared with the PTC1/PTC2-RNAi group.
Fig 2: Histomorphology of HE-stained spinal tissues in the seven groups. Notes: Gli-1, glioma-associated oncogene homolog 1; HE, hematoxylin and eosin; NC, negative control; PTC1, Patched 1; PTC2, Patched 2; SCI, spinal cord injury; Shh, sonic hedgehog.
Fig 3: Comparisons of PTC1 and PTC2 mRNA expression in DRG cells among the normal, PTC1-RNAi, PTC2-RNAi and PTC1/PTC2-RNAi groups. Notes: (a) relative PTC1 mRNA expression in DRG cells among the four groups; (b) relative PTC2 mRNA expression in DRG cells among the four groups; PTC1, Patched 1; PTC2, Patched 2; DRG, dorsal root ganglion; *P<0.05 compared with the normal group.
Fig 4: Comparison of the relative expression of PTC1, PTC2, Shh, Gli-1, Nestin and Smo proteins among the seven groups on the 7th, 14th and 28th days. Notes: (a) relative expression of PTC1, PTC2, Shh, Gli-1, Nestin and Smo proteins on the 7th day; (b) relative expression of PTC1, PTC2, Shh, Gli-1, Nestin and Smo proteins on the 14th day; (c) relative expression of PTC1, PTC2, Shh, Gli-1, Nestin and Smo proteins on the 21st day; (d) relative expression of Shh protein; (e) relative expression of Gli-1 protein; (f) relative expression of Smo protein. Relative expression of Nestin protein; (h) relative expression of PTC1 protein; (i) relative expression of PTC2 protein; Gli-1, glioma-associated oncogene homolog 1; NC, negative control; PTC1, Patched 1; PTC2, Patched 2; SCI, spinal cord injury; Shh, sonic hedgehog; *P<0.05 compared with the normal group; #P<0.05 compared with the SCI group; &P<0.05 compared with the PTC1/PTC2-RNAi group.
Fig 5: Representations of images of cells transfected with PTC1-RNAi, PTC2-RNAi and PTC1/PTC2-RNAi plasmids using fluorescence microscopy (× 200). Notes: (a) cells transfected with the pEGFP-PTC1 plasmid; (b) cells transfected with the pEGFP-PTC2 plasmid; (c) cells transfected with the pEGFP-PTC1/2 plasmid; (d) cells co-transfected with pEGFP-PTC1+ReteoQ-shRNA-1-PTC1 plasmids; (e) cells co-transfected with pEGFP-PTC2+ReteoQ-shRNA-1-PTC2 plasmids; (f) cells co-transfected with pEGFP-PTC1/2+ReteoQ-shRNA-1-PTC1/2 plasmids; (g) cells co-transfected with pEGFP-PTC1+ReteoQ-shRNA-2-PTC1; (h) cells co-transfected with pEGFP-PTC2+ReteoQ-shRNA-2-PTC2 plasmids; (i) cells co-transfected with pEGFP-PTC1/2+ReteoQ-shRNA-2-PTC1/2 plasmids; EGFP, enhanced green fluorescent protein; PTC1, Patched 1; PTC2, Patched 2.
Supplier Page from Proteintech Group Inc for PTCH2 antibody