Fig 1: Identification of the quality of EVs isolated by three methods. (A) EVs isolated by UC, PEG, and SEC methods were stained with PKH67 (100 μM) and observed with an Olympus FV3000 confocal microscope (n = 10). Bar = 5 μm. (B) The differential protein concentrations isolated by the three methods were normalized and compared with silver staining, and the differential protein concentrations of EVs CD63, HSP70, TSG101, and calnexin were observed by WB. (C) Representative WB images showed the expression of albumin, IgM, IgG, ApoA1, ApoA4, and ApoE.