Fig 2: Expression and methylation of RASSF1A in the skin lesions and effect of RASSF1A-overexpressing lentivirus infection and methylation inhibitor 5-Aza-CdR. A, Immunohistochemistry of RASSF1A in psoriasis and normal skin tissues (bar length = 600 µm). B, RASSF1A mRNA expression in psoriasis and normal skin tissues (n = 3). C, RASSF1A protein expression in psoriasis and normal skin tissues. D, RASSF1A methylation in psoriasis and normal skin tissues. E, RASSF1A, YAP, pS127-YAP, LATS1/2, p-LATS1/2, MST1/2 and p-MST1/2 protein expression after RASSF1A overexpression lentivirus transfection. F, RASSF1A methylation after adding methylation inhibitor 5-Aza-CdR. G, RASSF1A, YAP, pS127-YAP, LATS1/2, p-LATS1/2, MST1/2 and p-MST1/2 protein expression in M5-induced psoriatic cells after adding 5-Aza-CdR or RASSF1A overexpression lentivirus. M = methylated RASSF1A, U = unmethylated RASSF1A. BLK = empty lentivirus-transfected group. RASSF1A = RASSF1A overexpression lentivirus-transfected group. ***P < .001 vs Normal