Fig 1: RA significantly induces ABCG5/8 and ABCA1 protein levels, which are involved in hepatobilial cholesterol excretion, and CYP7A1 protein levels, a key rate-limiting enzyme for bile acid biosynthesis in HFD-fed mice. (A–D) ABCG5, ABCG8, ABCA1, and (E,F) CYP7A1 protein levels were examined in liver tissues by western blot analysis, and the relative protein levels are presented as the mean ± SD (n = 4–6). ** p < 0.01 vs. ND control mice; # p < 0.05, ## p < 0.01, vs. HFD mice; $ p < 0.05, $$ p < 0.01 vs. RA 50.