Fig 1: T-2 toxin exposure induced increase in the apoptosis-related protein expression by mitochondria-mediated pathway in HL-7702 cells. The mRNA and protein expressions of apoptosis-related proteins (Bax, Bcl-2, caspase 3, caspase 7, and caspase 9) were respectively evaluated after treatment with 0, 0.1, 1.0, and 10 µg/L T-2 toxin for 24 h. (A) increase and decrease in the gene levels of Bax and Bcl-2, respectively, relative to control; (B) increase in gene levels of caspase 3, caspase 7, and caspase 9 relative to control; (C) increase and decrease in the protein levels of Bax and Bcl-2, respectively, relative to control; (D) increase of protein levels of caspase 3, caspase 7, and caspase 9 relative to control. ß-actin was used as a reference gene. All values are presented mean ± SEM from three different experiments. * p <0.05; ** p <0.01 vs. control values.