Fig 1: Inflammation and fibrosis in the heart of shift worker rats. A, B Left: Illustration of CD45 (A) and CD68 (B) immunostaining in the heart of shift worker rats. Arrows indicate immuno-positive cells. Right: Quantification of the number of CD45+ (A) and CD68+ (B) in the heart of control, W, and WRF rats (n=3 per group; mean +/- s.e.m). Groups with different letters are significantly different (one-way ANOVA; p < 0.05). C Illustration of collagen 1 immunostaining (left) and quantification of collagen 1 signal (right) in the heart of control, W, and WRF rats (n=3 per group; mean +/- s.e.m). Groups with different letters are significantly different (one-way ANOVA; p < 0.05). D Left: representative picrosirius red staining in the heart of control, W, and WRF rats. Right: quantification of the picrosirius red staining (n=18 per group). Quantification was performed using a scale from 0 to 4 (0 = no staining, 4 = maximum staining; see Additional file 2: Fig. S8). Each dot corresponds to the average score given by four investigators for each sample. Groups with different letters are significantly different (one-way ANOVA; p < 0.05)