Fig 2: Receptor density.A) A schematic illustration of how temporal changes in receptor region area () and intensity are reflected. B) During the time period, both receptor intensity and area increase. The blue dashed line illustrates the isodensity line at T2. slopes greater than this isodensity line (i.e., illustrated by the green shaded area) represent intensity and area changes resulting in increasing density over this time period. Conversely, slopes less than the isodensity line (i.e., illustrated by the purple shaded area) would result from decreasing density over this time period. C–H) Distribution patterns of change in contact site area and intensity for DC-SIGN (C,E,G) and CD206 (D,F,H). Diamonds denote colocalized regions. Arrows annotate the temporal connection of the datapoints: blue for colocalized receptor regions. Time step arrows labeled “A” exhibit a significant change in area at . Time step arrows labeled “I” exhibit a significant change in intensity at . Dashed lines (nearly on top of the Area axes in (C–H) indicate the isodensity lines for zero hour colocalized datapoints. Statistical significance was determined by ANOVA and Tukey's post-hoc test with samples per donor for 1 and 4 hours and samples per donor for 0 h.

Fig 3: Human immature dendritic cells (DCs) form complex three dimensional host-pathogen contacts between DC plasma membrane (gray) and Candida albicans yeast (position depicted by blue wireframe sphere).Fungal recognition involves the deployment of C-type lectins such as DC-SIGN (green) and CD206 (red) to these contact sites. These contact sites have typical dimensions that match the diameter of C. albicans yeast, in the range of diameter.

Fig 4: Receptor area distribution patterns.A,B) Comparison of changes in total area, as a fraction of the entire contact as defined by the plasma membrane, for (A) DC-SIGN and (B) CD206 at time points post exposure to yeast (0, 1, 4 hours). C,D) Statistical comparison of total receptor percent area over the entire contact site for (C) DC-SIGN and (D) CD206 at one hour post exposure. E,F) Statistical comparison of total receptor percent area over the entire contact site for (E) DC-SIGN and (F) CD206 at four hours post exposure. G,H,I) Comparison of area distribution patterns by change in area over the entire contact site for (G) Colocalized receptors, (H) single positive DC-SIGN, and (I) single positive CD206 at time points post exposure to yeast (0, 1, 4 hours). Statistical significance was determined by ANOVA, Tukey post-hoc test (*** and “A”: ; letter colors match to figure legend) with samples per donor for 1 and 4 hours and samples per donor for 0 hour. Comparisons are between the designated point and other time points of the same color (A,B,G,H,I) or between the different species of yeast (C,D,E,F). Values and error bars displayed in all panels are presented as means and standard deviations.

Fig 5: Receptor intensity distribution patterns.A) Schematic representation of a model host-microbe contact site representing spatial distributions of DC-SIGN and CD206 intensity on the curved contact site membrane as a function of their spherical coordinates () relative to the yeast center. The dashed line depicts the boundary of the contact site as defined by DC plasma membrane stain present at the fungal cell wall edge. Within the contact area, there are regions of receptor colocalization and single receptor localization. B,C) Comparison of receptor total intensity over the entire contact for (B) DC-SIGN and (C) CD206 at times post exposure to yeasts (0, 1, 4 hours). D,E) Statistical comparison of total receptor intensity over the entire contact site for DC-SIGN at (D) one hour and (E) four hours post exposure. F,G) Statistical comparison of total receptor intensity over the entire contact site for CD206 at (F) one hour and (G) four hours post exposure. Statistical significance was determined by ANOVA, Tukey post-hoc test (*** and “A”: ; letter colors matched to figure legend) with samples per donor for 1 and 4 hours and samples per donor for 0 h. Comparisons are between the designated point and other time points of the same color (B,C) or between the different species of yeast (D,E,F,G). Values and error bars displayed in all panels are presented as means and standard deviations.