Fig 1: DDX6 granule assembly was regulated by translational activity. (A) Quantification of the mean number of DDX6 granules per cell before (NI) and after treatment of the hASCs with o-propargyl puromycin (OPP) (NI+OPP). Seventy cells per condition were analyzed (n = 70). Quartile 10–90%; Mann-Whitney test: **** p < 0.0001. (B) Images from immunofluorescence analysis by confocal microscopy of TIAR and DRIP localization in the hASCs treated with OPP only (30-min OPP) or OPP and sodium arsenite (30-min Ars/OPP). SGs containing TIAR showed accumulated DRIPs but only under stress conditions. White arrows: granules. Nuclei were stained with DAPI. (C) Results from immunofluorescence analysis by confocal microscopy of DDX6 and DRIP localization in the hASCs treated with OPP only (30 min of OPP incubation) or OPP and sodium arsenite (30 min of Ars/OPP incubation). DDX6 granules were found under both stress and nonstress conditions; however, they accumulated DRIPs only after stress induction. White arrows: granules. Nuclei were stained with DAPI. (D) Quantification of DDX6 granules enriched with DRIPs in the hASCs treated with only OPP (OPP) or with OPP and sodium arsenite (OPP+Ars30). The bar graph shows the percentages of DDX6 granules enriched with DRIPs (granule/surrounding region signal ratio > 1.5) per cell. At least 34 cells were analyzed per condition; standard error of the mean (SEM); Mann-Whitney test: **** p < 0.0001. (E) Quantification of TIAR granules enriched with DRIPs in the hASCs treated with only OPP (OPP) or OPP and sodium arsenite (OPP+Ars30). The cells treated with only OPP did not have assembled TIAR granules. The bar graph shows the percentages of TIAR granules enriched with DRIPs (granule/surrounding region signal ratio > 1.5) per cell. Thirty-two cells were analyzed; standard error of the mean (SEM).