Anti Iba1, Goat 011-27991 from FUJIFILM Wako Chemicals U.S.A. Corp

Product Specs
ItemAnti Iba1, Goat
CompanyFUJIFILM Wako Chemicals U.S.A. Corp
Price Supplier Page
Catalog Number011-27991
ClonalityPolyclonal Antibody
FormatLiquid
ApplicationsIHC(F), IHC(P), WB
HostGoat
Quantity100 µL
TargetIba1
Conjugate/TagUnconjugated
ReactivityMouse, Rat

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FUJIFILM Wako Chemicals U.S.A. Corp

1600 Bellwood Road

Richmond, VA 23237-1326

United States

Phone: 804-271-7677
Fax: 804-271-7791

Email: wkuslabchem@fujifilm.com

Company Profile

Website: www.wakousa.com

(1) INPP5D regulates inflammasome activation in human microgliaNature CommunicationsNovember 29, 2023Vicky Chou, Richard V. Pearse, Aimee J. Aylward, Nancy Ashour, Mariko Taga, Gizem Terzioglu, Masashi Fujita, Seeley B. Fancher, Alina Sigalov, Courtney R. Benoit, et al.6 μm sections of formalin-fixed paraffin-embedded tissue were immunostained for IBA1 (1:100, Wako, 011-27991), INPP5D (1:100, abcam, ab45142) and Aβ42 (1:300, Biolegend, 805501). Alternate brain sections were

(2) Qi Fu Yin ameliorates neuroinflammation through inhibiting RAGE and TLR4/NF-κB pathway in AD model ratsAging (Albany NY)November 22, 2023Chunxiang He, Wenjing Yu, Miao Yang, Ze Li, Jingping Yu, Dayuan Zhong, Sisi Deng, Zhenyan Song, Shaowu Chengdeparaffinized, the tissues were incubated overnight at 4°C with anti-NF-κB (bs-0465R, Bioss) and anti-Iba1(011-27991, Wako) primary antibodies. The following day, the samples were washed with PBS and incubated with fluorescent

(3) Differential Effects of Regulatory T Cells in the Meninges and Spinal Cord of Male and Female Mice with Neuropathic PainCellsSeptember 20, 2023Fiore NT, Keating BA, Chen Y, Williams SI, Moalem-Taylor G.diluted in PBS containing 2% NDS and 0.1% Triton X-100 overnight at 4 °C; goat anti-mouse IBA-1 (WAKO 011-27991 1:200), rabbit anti-mouse lysozyme (LYZ, Abcam ab108508 1:200), goat anti-mouse CD206 (RnDSystems AF2535

(4) Live imaging of microglia during sleeping sickness reveals early and heterogeneous inflammatory responsesFrontiers in ImmunologySeptember 13, 2023Nestor L. Uzcategui, Sena Güçer, Cris Richter, Annika Speidel, Elizabeta Zirdum, Michael Duszenko, Olga Garaschuk, Katherine Figarelladiluted in PBS-plus (containing 5% normal donkey serum and 1% triton): Iba-1 (1:500, goat; Wako, no. 011-27991), TMEM119 (1:200, rabbit; Abcam, no. ab209064). In the case of TMEM119 staining, an additional step

(5) Transplantation of Chemical Compound-Induced Cells from Human Fibroblasts Improves Locomotor Recovery in a Spinal Cord Injury Rat ModelInternational Journal of Molecular SciencesSeptember 8, 2023Toshihiro Kurahashi, Chiyoko Nishime, Eiko Nishinaka, Yuji Komaki, Fumiko Seki, Koji Urano, Yoshinori Harada, Toshikazu Yoshikawa, Ping Daiacetyltransferase (ChAT, AB144P, MerckMillipore, Burlington, MA, USA), the macrophage marker Iba1 (011-27991, Wako, Osaka, Japan), and the astrogliosis marker GFAP (G3893, Sigma-Aldrich, St. Louis, MO, USA). Signals

(6) Extracellular RNAs-TLR3 signaling contributes to cognitive impairment after chronic neuropathic pain in miceSignal Transduct Target TherAugust 7, 2023Xueying Zhang, Rui Gao, Changteng Zhang, Yi Teng, Hai Chen, Qi Li, Changliang Liu, Jiahui Wu, Liuxing Wei, Liyun Deng, et al.Proteintech), anti-GFAP mouse antibody (1:200, 60190-1-Ig, Proteintech), anti-IBA1 goat antibody (1:100, 011-27991, WAKO) and anti-dsRNA mouse antibody J2 (1:200, 10010500, SCICONS). After overnight incubation with

(7) High-Fat Diet Consumption Induces Neurobehavioral Abnormalities and Neuronal Morphological Alterations Accompanied by Excessive Microglial Activation in the Medial Prefrontal Cortex in Adolescent MiceInternational Journal of Molecular SciencesMay 28, 2023Conghui Wang, Hong Li, Chen Chen, Xiuting Yao, Chenxi Yang, Zhehao Yu, Jiayi Ren, Yue Ming, Yi Huang, Yi Rong, et al.anti-CD68 (for phagocytic microglia, 1:1000, MCA1957; Bio-Rad, Oxford, UK), goat anti-Iba1 (1:600, 011–27991; Wako), and rabbit anti-PSD95 (for the postsynaptic element, 1:1000, ab18258; Abcam, Cambridge, UK).

(8) CXCL13 contributes to chronic pain of a mouse model of CRPS-I via CXCR5-mediated NF-κB activation and pro-inflammatory cytokine production in spinal cord dorsal hornJournal of neuroinflammationMay 8, 2023Wang J, Yin C, Pan Y, Yang Y, Li W, Ni H, Liu B, Nie H, Xu R, Wei H, et al.1:300, mouse monoclonal, Abcam), GFAP antibody (#3670s, 1:200, mouse monoclonal, CST), Iba1 antibody (#011-27991, 1:500, goat polyclonal, Wako). The sections were then incubated 1 h at 37 °C with corresponding secondary

(9) Increased neutrophils in inflammatory bowel disease accelerate the accumulation of amyloid plaques in the mouse model of Alzheimer’s diseaseInflammation and RegenerationMarch 15, 2023Ryusei Kaneko, Ako Matsui, Mahiro Watanabe, Yoshihiro Harada, Mitsuhiro Kanamori, Natsumi Awata, Mio Kawazoe, Tomoaki Takao, Yutaro Kobayashi, Chie Kikutake, et al.antibodies were used: anti-human Aβ (1:200; 82E1, IBL), anti-mouse IBA1 (goat polyclonal antibody, 1:100; 011-27991, WAKO), anti-mouse GFAP (rabbit polyclonal antibody, 1:100 for cryosections, 1:500 for paraffin sections;

(10) P2X7 receptor-activated microglia in cortex is critical for sleep disorder under neuropathic painFrontiers in NeuroscienceFebruary 3, 2023Tingting Li, Yunling Gao, Mengying He, Zhu Gui, Bingchu Zhao, Yue Cao, Ting Chen, Jinpiao Zhu, Jie Wang, Qi Zhong, et al.following primary antibodies: P2X7R (1:200, rabbit, APR-004, Alomone, USA) and Iba-1 (1:200, goat, 011-27991, Wako, UK). Then, they were then washed and incubated with appropriate fluorescent secondary antibodies

(11) DSCR1 deficiency ameliorates the Aβ pathology of Alzheimer's disease by enhancing microglial activityLife science allianceFebruary 1, 2023Choi C, Kim H, Oh J, Park C, Kim M, Kim CS, Park J.(1:500, NBP2-13075; Novus), TREM2 (1: 100, MAB17291-100; R&D Systems), and Iba1 (1:500, 019-19741, 011-27991; Wako) primary antibodies with 2% normal horse serum in PBS-T at 4°C overnight. After 1-d incubation,

(12) AdipoRon Engages Microglia to Antinociception through the AdipoR1/AMPK Pathway in SNI MiceMediators of inflammationJanuary 1, 2023Fang Q, Li J, Wang Y, Liu X, Shi Y, Chen J, Zhan H, Zeng Y, Wu W.incubated with primary antibodies against AdipoR1 (1 : 1000, Abcam, ab126611), Iba-1 (1 : 800, Wako, 011-27991), GFAP (1 : 300, Cell Signaling Technology, #3670), and NeuN (1 : 800, Millipore, MAB377) for 16-20

(13) Molecular and anatomical roadmap of stroke pathology in immunodeficient miceFrontiers in ImmunologyDecember 9, 2022Rebecca Z. Weber, Geertje Mulders, Patrick Perron, Christian Tackenberg, Ruslan Rustincubated with primary antibodies (rabbit anti-GFAP 1:200, Dako, #GA524; goat anti-Iba1, 1:500 Wako, #011-27991; NeuroTrace™ 1:200, Thermo Fischer; mouse anti-NeuN Antibody 1:500, Merck, #MAB377; rabbit anti-Neurofilament

(14) Opposing effects of apoE2 and apoE4 on microglial activation and lipid metabolism in response to demyelinationMolecular neurodegenerationNovember 23, 2022Wang N, Wang M, Jeevaratnam S, Rosenberg C, Ikezu TC, Shue F, Doss SV, Alnobani A, Martens YA, Wren M, et al.calcium-binding adaptor molecule 1 (Iba-1) antibody (1:1000, Wako, 19–19741); anti-Iba-1 antibody (1:200, Wako, 011–27991). For some experiments, frozen Sects. (40 μm thick) were subjected to immunofluorescence staining. The

(15) Loss of functional System x-c uncouples aberrant postnatal neurogenesis from epileptogenesis in the hippocampus of Kcna1-KO miceCell reportsNovember 22, 2022Aloi MS, Thompson SJ, Quartapella N, Noebels JL.Zogenix, Inc.), mouse-anti-GFAP (1:500; Millipore, IF03L), or rabbit-anti-IBA-1 (1:500, Wako Chemicals, 011-27991). Sections were washed with 0.01% Tween 20 in 1x PBS, incubated with Alexa Fluor–labeled secondary antibodies

(16) Optogenetic induction of hibernation-like state with modified human Opsin4 in miceCell Reports MethodsNovember 21, 2022Tohru M. Takahashi, Arisa Hirano, Takeshi Kanda, Viviane M. Saito, Hiroto Ashitomi, Kazumasa Z. Tanaka, Yasufumi Yokoshiki, Kosaku Masuda, Masashi Yanagisawa, Kaspar E. Vogt, et al.(1:15,000, AB0040-200, SICGEN), rat anti-GFP (1:5,000, 04,404-84, Nacalai Tesque), goat anti-Iba1 (1:100, 011–27991, WAKO) and rabbit anti-GFAP (1:2000, HPA056030, ATLAS ANTIBODIES). Secondary antibodies were Alexa Fluor

(17) The Protective Effect of CBD in a Model of In Vitro Ischemia May Be Mediated by Agonism on TRPV2 Channel and Microglia ActivationInternational Journal of Molecular SciencesOctober 12, 2022Daniele Lana, Elisa Landucci, Costanza Mazzantini, Giada Magni, Domenico Edoardo Pellegrini-Giampietro, Maria Grazia Giovanniniantibody to immunostain the neurons (1:400 in BB), and a goat anti-IBA1 antibody (1:200, product code: 011-27991, WAKO, Osaka, Japan). Day 2. After extensive washing, the slices were incubated for 2 h at room temperature

(18) Reactive Oxygen Species Contributes to Type 2 Diabetic Neuropathic Pain via the Thioredoxin-Interacting Protein-NOD-Like Receptor Protein 3- N -Methyl-D-Aspartic Acid Receptor 2B PathwayAnesthesia and analgesiaOctober 1, 2022Wang JW, Ye XY, Wei N, Wu SS, Zhang ZH, Luo GH, Li X, Li J, Cao H.antibody (1:100; MAB377, Millipore), ionized calcium-binding adaptor protein (Iba) antibody (1:500; 011-27991, Wako) or a mixture of NLRP3 antibody (1:50; M035175, Abmart) and TXNIP antibody (1:50;T572136, Abmart).

(19) Single cell RNA sequencing confirms retinal microglia activation associated with early onset retinal degenerationScientific ReportsSeptember 10, 2022Asha Kumari, Raul Ayala-Ramirez, Juan Carlos Zenteno, Kristyn Huffman, Roman Sasik, Radha Ayyagari, Shyamanga Borooahat 4 °C. Further, for APOE (rabbit anti-mouse, 1:100, ab183597) and Iba-1 (goat anti-mouse, 1:500, 011-27991,Wako chemicals) co-staining and for TMEM119 rabbit anti-mouse, 1:500 ab209064), and CD68 (rat-anti mouse,

(20) Alteration of m6A epitranscriptomic tagging of ribonucleic acids after spinal cord injury in miceFrontiers in NeuroscienceAugust 25, 2022Shuangfei Ni, Zixiang Luo, Yonggang Fan, Weixin Zhang, Wei Peng, Huafeng Zhang(R&D Systems, Inc., FAB3628G-100, and 1:200), anti-GFAP (Abcam, ab53554, and 1:500), anti-Iba-1 (Wako, 01127991, and 1:800) were incubated at 4°C overnight. The corresponding secondary antibodies (Abcam and 1:500)

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Anti Iba1, Goat from FUJIFILM Wako Chemicals U.S.A. Corp

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FUJIFILM Wako Chemicals U.S.A. Corp

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Anti Iba1, Goat from FUJIFILM Wako Chemicals U.S.A. Corp

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