Fig 1: Hyperoxia induced ILC2 and IL-6+ILC2 in BPD mice. A Representative FACS analysis showing the gating strategy used to identify CD45+Lin-CD90.2+Sca-1+ILC2 in lung of BPD mice. B, C Representative results and percentage of ILC2 detected by flow cytometry in lung tissues from the normoxia and hyperoxia groups. D Representative Wright-Giemsa staining of sorted ILC2. E, F Representative results and percentage of IL-6+ILC2 (gate: CD45+Lin-CD90.2+Sca-1+) detected by flow cytometry in normoxia and hyperoxia groups. G RT-qPCR analysis of IL-6 mRNA levels in ILC2 of normoxia and hyperoxia groups. H ELISA analysis of IL-6 expression in the blood serum and lung tissues from the normoxia and hyperoxia groups. Data represented as mean ± SD (n = 6); ** P < 0.05 vs. normoxia group