Fig 1: E. multilocularis infection promotes colonic Treg expansion.On day 90 post-infection of Emu, the mice were euthanized and subject to flow cytometry analysis of colonic (lamina propria) and splenic lymphocytes. a Expression of CD25 by immune cells isolated from the colon of naive and Emu-infected mice (left), and proportions of CD25+ T cells in CD3+CD4+ cells in colon or spleen (right). b Expression of CD25 and Foxp3 by immune cells isolated from the lamina propria of the colon in naive and Emu-infected mice (left), and the proportion of colonic CD25+Foxp3+ T cells in the CD3+CD4+ cell population (right). c The proportion of colonic CD25-Foxp3+ T cells in the CD3+CD4+ cell population. d Expression of CD103 and Foxp3 by immune cells isolated from the lamina propria of the colon in naive and Emu-infected mice (left), and the proportion of colonic CD103+Foxp3+ T cells in the CD3+CD4+ cell population in LP (right). Flow cytometry data are representative of n = 7 mice per group. Experiments were repeated 2 times with similar results. The data shown are from a representative experiment (mean ± SD shown). ns, not significant. *P < 0.05, **P < 0.01, using the two-sided Student t-test.