Fig 1: Effects of Rab43 knockout on the cell surface transport of endogenous CD91. (A) The CD91 proteins on the cell surface were analyzed using flow cytometry. The bone marrow-derived macrophages (BMDMs) were isolated from Rab43-C and Rab43-cKO mice. *P < 0.05 versus the Rab43-C group. (B) The CD91 levels on the cell surface of BALF samples. The Rab43-C and Rab43-cKO mice were treated with LPS for 1 (upper panel) and 3 days (lower panel). The macrophages (F4/80+CD11c+CD11b-) in the BALF samples were also analyzed. *P < 0.05 versus the Rab43-C group. (C) The expression of total, cytoplasmic, and membrane CD91 in Rab43-C and Rab43-cKO BMDMs measured using western blot analyses. *P < 0.05 versus Rab43-C group. ns, not statistically significant.