Fig 1: PRR7 inhibits synaptogenic Wnt signaling. a, b Effect of exogenous application of Wnt5a and Wnt7a on PRR7-induced loss of PSD-95 clusters. Representative immunofluorescent images of PSD-95 (a) and quantitation of data (b). n = 23–26. c, d Effect of soluble Wnt inhibitors on PRR7 knockdown-induced increase of PSD-95 clusters. Representative immunofluorescent images of PSD-95 (c) and quantitation of data (d). n = 22–24. e, f Modulation of Wnt signaling by PRR7. Representative immunoblots showing the effect of PRR7 overexpression or knockdown on the indicated Wnt signaling components (e) and quantification of the data (f). n = 3–5. g Effect of PRR7 knockdown on the exosomal secretion of Wnt5a and Wnt7a. n = 4. Data are mean ± SEM. Scale bars, 5 µm. Two-way ANOVA (b, d), post hoc Tukey’s test: F2,148 = 66.62 (b, GFP), F2,138 = 76.96 (b, myc-PRR7), F2,130 = 86.74 (d, Ctrl RNAi), and F2,134 = 336.6 (d, PRR7 RNAi). ***P < 0.0001; Student t test (f, g) and *P < 0.05. Mr relative molecular weight