Fig 1: Quantitative PCR shows physiological expression of Nhlrc1 in four tissues from FLAG-malin mice, with no alterations in expression of Epm2a, Gys1 (glycogen synthase) and Gbe1 (glycogen branching enzyme). (A-D) Total RNA from indicated tissues (3 months, males and females equally distributed with n=8) were extracted and analyzed for quantification of expression of the indicated genes. Values are expressed as means±s.d.